质量水平
用途
kit sufficient for 100 assays (1 mL)
运输
dry ice
储存温度
−20°C
基因信息
human ... TXNRD1(7296), TXNRD2(10587), TXNRD3(114112)
一般描述
硫氧还蛋白还原酶检测试剂盒采用比色法对哺乳动物细胞核组织中的硫氧还蛋白还原酶进行检测。该试剂盒原理是硫氧还蛋白还原酶可催化NADPH使5,5′-二硫双(2-硝基苯甲酸) (DTNB)还原成5-硫代-2-硝基苯甲酸(TNB)，后者在412nm处呈现强黄色。
DTNB + NADPH + H+ ↔ 2 TNB + NADP+
DTNB + NADPH + H+ ↔ 2 TNB + NADP+
应用
使用大鼠肝去除细胞核后的提取物，本试剂盒的线性度可达0.25单位/ml。在该试剂盒反应中，约20%的活性是非特异性还原酶的活性。(TrxR抑制剂对其无效).
特点和优势
- 检测方法简单易行。
- 试剂盒中包含了比色法检测哺乳动物硫氧还蛋白还原酶所需的所有试剂。
- 试剂盒中提供了特异性抑制哺乳动物硫氧还蛋白还原酶的溶液。
- 该试剂盒已通过心肌和HeLa, A549, Jurkat, U937, A431, COS, CHO 和 NIH 3T3 等细胞系裂解物的测试。
仅试剂盒组分
产品编号
说明
- Assay Buffer 5x for Thioredoxin Reductase 30 mL
- Thioredoxin Reductase 10 μg
- Thioredoxin Reductase Inhibitor Solution .05 mL
- 5,5'-Dithiobis(2-nitrobenzoic) acid (DNTB) 150 mg
- NADPH 25 mg
- Dimethyl Sulfoxide (DMSO) 7.5 mL
警示用语：
Warning
危险声明
危险分类
Repr. 2
储存分类代码
10 - Combustible liquids
闪点(°F)
188.6 °F
闪点(°C)
87 °C
法规信息
低风险生物材料
动植物源性产品
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