应用
该试剂盒既可用于检测DHFR活性，也可用于筛选和DHFR抑制剂。检测原理为NADPH的DHFR能够可逆催化二氢叶酸还原为四氢叶酸，再通过监测340nm处吸光度降低情况就可以跟踪反应进程。
二氢叶酸+ NADPH+H+↔四氢叶酸+NADP+
二氢叶酸+ NADPH+H+↔四氢叶酸+NADP+
生化/生理作用
DHFR（二氢叶酸还原酶）是一种在核苷酸碱基反应中催化生物合成DNA所必需的酶。阻断该酶就会抑制DNA合成，最终导致细胞死亡。因此DHFR也是抗肿瘤药物的出色靶点。
特点和优势
- 操作方法快速、简便。
- 包含比色法测定DHFR活性所需的全部试剂，适用于细胞裂解物、组织匀浆或酶纯化柱组分。
- 包含纯化酶，可用作阳性对照或用于筛选DHFR抑制剂。
- 包含甲氨蝶呤（MTX），后者是一种原核和真核的DHFR特异性抑制剂，具有抗肿瘤活性。
- 已经在A431、NIH-3T3、CHO细胞系，大鼠肝、肾、脑和骨骼肌组织提取物，以及重组DHFR中进行测试。
仅试剂盒组分
产品编号
说明
- Assay Buffer 10x for DHFR 30 mL
- Dihydrofolate Reductase (DHFR) human .1 U
- Dihydrofolic acid (DHFR substrate) 3 x 10
- Amethopterin (+)(methotrexate, MTX)
(DHFR inhibitor) 2 x 10 - NADPH (β-Nicotinamide adenine dinucleotide phosphate reduced tetrasodium salt) 25 mg
警示用语：
Danger
危险分类
Acute Tox. 3 Oral - Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
此项目有
Marta Jorba et al.
Antibiotics (Basel, Switzerland), 10(6) (2021-07-03)
This work reports a detailed characterization of the antimicrobial profile of two trimethoprim-like molecules (compounds 1a and 1b) identified in previous studies. Both molecules displayed remarkable antimicrobial activity, particularly when combined with sulfamethoxazole. In disk diffusion assays on Petri dishes
Bharath Srinivasan et al.
European journal of medicinal chemistry, 103, 600-614 (2015-09-29)
Gram-negative bacteria are implicated in the causation of life-threatening hospital-acquired infections. They acquire rapid resistance to multiple drugs and available antibiotics. Hence, there is the need to discover new antibacterial agents with novel scaffolds. For the first time, this study
Marcus S Bray et al.
Proceedings of the National Academy of Sciences of the United States of America, 115(48), 12164-12169 (2018-11-11)
Today, Mg2+ is an essential cofactor with diverse structural and functional roles in life's oldest macromolecular machine, the translation system. We tested whether ancient Earth conditions (low O2, high Fe2+, and high Mn2+) can revert the ribosome to a functional
Amrinder Singh et al.
Scientific reports, 8(1), 3190-3190 (2018-02-18)
We report the first peptide based hDHFR inhibitors designed on the basis of structural analysis of dihydrofolate reductase (DHFR). A set of peptides were rationally designed and synthesized using solid phase peptide synthesis and characterized using nuclear magnetic resonance and
Bharath Srinivasan et al.
The FEBS journal, 282(10), 1922-1938 (2015-02-24)
Dihydrofolate reductase (DHFR) is a pivotal enzyme involved in the de novo pathway of purine synthesis, and hence, represents an attractive target to disrupt systems that require rapid DNA turnover. The enzyme acquires resistance to available drugs by various molecular
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