Anti-Mouse F4/80 (BM8.1) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

Adhesion G protein-coupled receptor E1 (UniProt: Q61549; also known as Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor) is encoded by the Adgre1 (also known as Emr1, Gpf480) gene (Gene ID: 13733) in murine species. F4/80 is a multiple-pass membrane protein that is synthesized with a signal peptide (aa 1-27), which is subsequently cleaved off to generate the mature form that contains four extracellular domains, seven transmembrane domains, and four cytoplasmic domains. The extracellular domain contains repeated EGF-like calcium binding domains. F4/80 serves as an orphan receptor and is involved in cell adhesion and cell-cell interactions in the immune system. It is also involved in the development of regulatory T-cells (Tregs). Mice deficient inF4/80 fail to develop peripheral tolerance after inoculation with antigen because of a lack of efferent regulatory T-cell development. Its expression is reported to vary amongst mouse mononuclear populations, being very low or absent from osteoclasts and lung alveolar macrophages. High expression of F4/80 has been proposed as a marker for populations of mouse tissue macrophages that derive from embryonic progenitors and renew independently of blood monocytes. (Ref.: Waddell, LA., et al. (2018). Front. Immunol. 9; 2246).

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Murine macrophages.

Quality Control Testing:

Evaluated by Flow Cytometry in Mouse bone marrow cells.

Flow Cytometry Analysis: Staining of one million mouse bone marrow cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1005, Anti-Mouse F4/80 (BM8.1) ColorWheel^® Dye-Ready mAb and Cat. No. CW-PE ColorWheel^® activated Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Rat IgG2b isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Clone BM8.1 is a rat monoclonal antibody that detects murine F4/80 (Adhesion G protein-coupled receptor E1). It targets an epitope within the extracellular domain.

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.