Anti-Mouse CD38 (90) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 (UniProt: P56528; also known as EC:3.2.2.6, 2′-phospho-ADP-ribosyl cyclase, 2′-phospho-ADP-ribosyl cyclase/2′-phospho-cyclic-ADP-ribose transferase, 2′-phospho-cyclic-ADP-ribose transferase, ADP-ribosyl cyclase 1, ADPRC 1, Cyclic ADP-ribose hydrolase 1, cADPr hydrolase 1, I-19, NIM-R5 antigen, CD38) is encoded by the Cd38 gene (Gene ID: 12494) in murine species. CD38, a member of the ADP-ribosyl cyclase family, is a single-pass type II membrane glycoprotein that contains a short cytoplasmic domain (aa 1-21), a transmembrane domain (aa 22-44), and a long extracellular domain (aa 45-304). It is expressed in many immune cell types, including bone marrow progenitors, natural killer cells, monocytes, and activated T- and B- lymphocytes. It serves as a lymphocyte receptor and is a considered to be a prognostic marker for survival with B-cell chronic lymphoblastic leukemia. The catalytic activity of CD38 resides in the extracellular domain that can both generate and degrade cyclic-ADP ribose and can catalyze the base-exchange reaction between NADP+ and nicotinic acid to produce NAADP+. Conformational change in CD38 can influence its catalytic activities. Clone 90 is reported to induce conformational change in CD38 and serves as agonistic antibody. It stimulates NAD+ glycohydrolase activity of FLAG-CD38 but has no effect on its cyclase activity. However, it inhibits the cyclase activity of cell surface CD38. (Ref.: Hartman, WR., et al. (2010). Leuk. Lymphoma. 51(7); 1315 1325l; Hara-Yokoyama, M., et al. (2008). Int. Immunopharmacol. 8(1); 59-70).

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Mouse bone marrow pre-B cells.

Quality Control Testing:

Evaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: Staining of one million mouse splenocytes was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1038, Anti-Mouse CD38 (90) ColorWheel^® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel^® activated Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Rat IgG2a isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Clone 90 is a rat monoclonal antibody that detects mouse ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 (CD28). It targets an epitope within the C-terminal region.

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.