Biochem/physiol Actions
真核生物的NADPH-细胞色素C还原酶（NADPH-细胞色素P450还原酶，EC1.6.2.4）是一种位于内质网的黄素蛋白。它可将NADPH的电子传递到负责对异型生物质发挥解毒功能的多种氧合酶，其中最重要的是细胞色素P450家族的酶。NADPH-细胞色素C还原酶被广泛用作内质网的标记蛋白，以及生态污染和膳食脂质摄取的生物标记物。
Features and Benefits
- 用于使用密度梯度分离法在分离和亚细胞分离时检测内质网(ER)
- 在药物中毒或外源性物质激发后，使用比色法检测多种来源的NADPH-细胞色素C还原酶
- 用于分析内质网的聚集及其功能
Analysis Note
细胞色素C还原酶检测试剂盒可快速便捷地检测细胞、组织提取物以及纯化的微粒体中的NADPH-细胞色素C还原酶活性，其含有该检测所需的所有试剂。该试剂盒已通过对肝、肾、脑、脾和心肌等多种哺乳动物组织样本以及HeLa、HepG2和Jurkat等细胞裂解物的测试。此外，还通过了对P. pastoris 和 S. cerevisiae.酵母菌株样本进行的测试。
仅试剂盒组分
产品编号
说明
- Assay Buffer 100 mL
- Enzyme Dilution Buffer 25 mL
- Cytochrome c 50 mg
- Cytochrome c Reductase (NADPH) Positive Control from rabbit liver 50 μL
- Cytochrome c Oxidase Inhibitor Solution 1 mL
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- N6505β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, ≥95% (HPLC) 25 mg
signalword
Danger
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
target_organs
Eyes,Central nervous system
wgk
WGK 3
hcodes
Hazard Classifications
Acute Tox. 3 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - STOT SE 2
法规信息
监管及禁止进口产品
此项目有
Protein and gene structure and regulation of NADPH-Cytochrome P450 oxidoreductase.
Shen, A. L. and Kasper, C. B. et al.
Handbook of Experimental Pharmacology, 105, 35-59 (1993)
A Hussain et al.
Human & experimental toxicology, 39(10), 1318-1332 (2020-04-30)
Various studies in rodents have shown that nanoparticles are transferred to the breast milk. Under the present study, lactating Wistar rats were repetitively gavaged 5, 25, and 50 mg/kg bw of zinc oxide nanoparticles (ZnO-NPs) and 50 mg kg-1 bw
Xiyan Li et al.
G3 (Bethesda, Md.), 9(2), 513-522 (2018-12-17)
Metformin elicits pleiotropic effects that are beneficial for treating diabetes, as well as particular cancers and aging. In spite of its importance, a convincing and unifying mechanism to explain how metformin operates is lacking. Here we describe investigations into the
Camila Ritter et al.
Molecular neurobiology, 57(5), 2420-2435 (2020-02-26)
Central neuropathic pain is a common untreated symptom in progressive multiple sclerosis (PMS) and is associated with poor quality of life and interference with patients' daily activities. The neuroinflammation process and mitochondrial dysfunction in the PMS lesions generate reactive species.
Seasonal variations of pollution biomarkers in two populations of Corbicula fluminea (Muller).
Vidal, M.-L., et al.
Comparative Biochemistry and Physiology, 131, Part C, 133-151 (2002)
商品
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。
亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。
相关内容
Instructions
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| CY0100-1KT | 04061826754184 |
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持