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Merck
CN

D0428

Sigma-Aldrich

1 kb DNA分子量标准

for DNA electrophoresis

别名:

1kb gel ladder, 1kb gel marker, 1kb ladder for gel electrophoresis, DNA gel marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker

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关于此项目

UNSPSC代码:
41105335
NACRES:
NA.25

主要文件

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质量水平

100
200

表单

liquid

适用性

suitable for electrophoresis (DNA)

储存温度

−20°C

相关类别

一般描述

Sigma的1kb分子量标准含有11个片段，包含0.5至3 kb的500bp重复序列，3至6 kb的1 kb重复序列，以及6至10 kb的2 kb重复序列。在核酸凝胶电泳中，应在凝胶缓冲液中稀释5μl分子量标准，然后在琼脂糖或聚丙烯酰胺凝胶上单泳道上样。适用于Northern和Southern印迹杂交。所有的DNA标记物都可以用溴化乙锭、SYBR® Green和Nancy-520染色。

应用

1 kb DNA分子量标准品已用作通过凝胶电泳测定双链DNA的分子量和大小的分子量标准。

其他说明

Sigma的1kb分子量标准品以溶于10mM Tris-HCl(pH 8.0)和1.0 mM EDTA的溶液形式提供。
为实现最佳分辨率，建议采用的琼脂糖凝胶浓度为0.75%。

法律信息

SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries

储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000278742
0000278742
SLCN2917
SLCK0031
SLCJ3543

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Luca Cocolin et al.
Applied and environmental microbiology, 70(3), 1347-1355 (2004-03-10)
In this paper we describe the development of a PCR protocol to specifically detect Brettanomyces bruxellensis and B. anomalus. Primers DB90F and DB394R, targeting the D1-D2 loop of the 26S rRNA gene, were able to produce amplicons only when the
W S Choe et al.
Biotechnology progress, 17(6), 1107-1113 (2001-12-12)
The direct chemical extraction of recombinant L1 protein (the major capsid protein of human papillomavirus type 16) from the cytoplasm of E. coli HMS174(DE3) has recently been demonstrated at high cell density (to OD(600) = 160) without the use of
Yu Zhou et al.
Journal of experimental botany, 59(10), 2803-2813 (2008-06-03)
The turnip crinkle virus-based vector TCV-GFP Delta CP had been devised previously to study cell-to-cell and long-distance spread of virus-induced RNA silencing. TCV-GFP Delta CP, which had been constructed by replacing the coat protein (CP) gene with a green fluorescent
Lidia Fuentes et al.
Journal of inorganic biochemistry, 203, 110875-110875 (2019-11-11)
One mononuclear and another dinuclear Pd(II) complexes bearing a α-N-heterocyclic thiosemicarbazone ligand have been synthesized, fully characterized and studied as biological agents. In both complexes, the palladium center is coordinated to 3,5-diacetyl-1,2,4-triazol bis(N4,N4-dimethylthiosemicarbazone) via three sites (N, N and S).
Ana I Matesanz et al.
Chembiochem : a European journal of chemical biology, 21(8), 1226-1232 (2019-11-21)
The synthesis and characterization of three new platinum complexes, with 3,5-diacetyl-1,2,4-triazole bis(4-N-isopropylthiosemicarbazone) as a ligand, are reported. The specific conditions under which solvent coordination takes place are reported and the X-ray structure of the complex with one solvent molecule of

商品

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

选择用于凝胶电泳的DNA、RNA和PCR片段的标记物和分子量标准

选择适合的标记物和分子量标准，用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。

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