D0572
杜氏改良 Eagle 培养基 - 高葡萄糖
AQmedia™, With 4500 mg/L glucose, 25mM HEPES, sodium bicarbonate and L-Ala-L-Gln, HEPES modification, liquid, sterile-filtered, suitable for cell culture
别名:
DME, DMEM
无菌性
sterile-filtered
产品线
AQmedia™
表单
liquid
技术
cell culture | mammalian: suitable
杂质
Endotoxin tested
组分
NaHCO3: 3.7 g/L
glucose: 4.5 g/L (Dextro)
L-glutamine: 0.584 g/L
phenol red: 0.0159 g/L
HEPES: 5.958 g/L
运输
ambient
储存温度
2-8°C
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一般描述
This DMEM-Hi glucose medium differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.
应用
Dulbecco′s Modified Eagle′s Medium（DMEM）是对Basal Medium Eagle (BME)的一种改良，含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此，它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养，以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外，葡萄糖浓度也被提高至4500 mg/L，因此被命名为“DMEM/High”。
法律信息
AQmedia is a trademark of Sigma-Aldrich Co. LLC
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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