sterility
sterile-filtered
product line
AQmedia™
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
Endotoxin tested
components
NaHCO3: 3.7 g/L
glucose: 4.5 g/L (Dextro)
L-glutamine: 0.584 g/L
phenol red: 0.0159 g/L
HEPES: 5.958 g/L
shipped in
ambient
storage temp.
2-8°C
General description
This DMEM-Hi glucose medium differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.
Application
Dulbecco′s Modified Eagle′s Medium（DMEM）是对Basal Medium Eagle (BME)的一种改良，含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此，它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养，以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外，葡萄糖浓度也被提高至4500 mg/L，因此被命名为“DMEM/High”。
Legal Information
AQmedia is a trademark of Sigma-Aldrich Co. LLC
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable