生物来源
Escherichia coli
质量水平
表单
aqueous glycerol solution
分子量
~374 kDa
浓度
≥2 unit/μL
技术
cell based assay: suitable
应用
cell analysis
运输
dry ice
储存温度
−70°C
基因信息
Escherichia coli K12 ... gyrA(946614), gyrB(948211)
应用
来自 大肠杆菌 的 DNA 旋转酶已用于研究比较蛋白质组学方法，以更好地定义拟核异常球菌的特异性。来自 大肠杆菌 的 DNA 旋转酶也用于研究DnaK-ClpB bichaperone系统在 DNA 旋转酶再激活中的作用。
生化/生理作用
DNA 旋转酶以A 2 B 2 全酶的形式提供。亚单位 A 的分子量为 97 kDa，亚单位 B 的分子量为 90 kDa。它催化负性超螺旋 ATP 依赖性引入松弛 DNA 这一过程。通过诱变实验，已成功地将 DNA 旋转酶转化为 II 类型拓扑异构酶。
可用于超螺旋质粒。
其他说明
在 37°C下，一个单位的旋转酶活性将在30分钟内超螺旋 0.5 微克的 pBR-322 DNA。
溶于含 Tris 缓冲液、DTT 和 EDTA 的 50% 甘油中。
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
此项目有
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