D0690
DNA Gyrase 来源于大肠杆菌
aqueous glycerol solution
产品名称
DNA Gyrase 来源于大肠杆菌, aqueous glycerol solution
biological source
Escherichia coli
form
aqueous glycerol solution
mol wt
~374 kDa
concentration
≥2 unit/μL
technique(s)
cell based assay: suitable
application(s)
cell analysis
shipped in
dry ice
storage temp.
−70°C
Quality Level
Gene Information
Escherichia coli K12 ... gyrA(946614), gyrB(948211)
Other Notes
溶于含 Tris 缓冲液、DTT 和 EDTA 的 50% 甘油中。
在 37°C下，一个单位的旋转酶活性将在30分钟内超螺旋 0.5 微克的 pBR-322 DNA。
Application
来自 大肠杆菌 的 DNA 旋转酶已用于研究比较蛋白质组学方法，以更好地定义拟核异常球菌的特异性。来自 大肠杆菌 的 DNA 旋转酶也用于研究DnaK-ClpB bichaperone系统在 DNA 旋转酶再激活中的作用。
Biochem/physiol Actions
DNA 旋转酶以A 2 B 2 全酶的形式提供。亚单位 A 的分子量为 97 kDa，亚单位 B 的分子量为 90 kDa。它催化负性超螺旋 ATP 依赖性引入松弛 DNA 这一过程。通过诱变实验，已成功地将 DNA 旋转酶转化为 II 类型拓扑异构酶。
可用于超螺旋质粒。
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Magali Toueille et al.
Journal of proteomics, 75(9), 2588-2600 (2012-03-27)
Compared to radiation-sensitive bacteria, the nucleoids of radiation-resistant Deinococcus species show a higher degree of compaction. Such a condensed nucleoid may contribute to the extreme radiation resistance of Deinococcus by limiting dispersion of radiation-induced DNA fragments. Architectural proteins may play
Alix Pantel et al.
Antimicrobial agents and chemotherapy, 56(4), 1990-1996 (2012-02-01)
Fluoroquinolone (FQ) resistance is emerging in Mycobacterium tuberculosis. The main mechanism of FQ resistance is amino acid substitution within the quinolone resistance-determining region (QRDR) of the GyrA subunit of DNA gyrase, the sole FQ target in M. tuberculosis. However, substitutions
Valentina Monica et al.
Lung cancer (Amsterdam, Netherlands), 79(3), 228-235 (2013-01-02)
Thymic epithelial tumors include several entities with different biologic behavior. Chemotherapy is indicated in advanced disease, but limited data exist on gene expression correlation with the response to chemotherapeutic agents. A series of 69 thymic neoplasms (7 A-, 6 AB-
PTTG1 expression and it's rapidly evolving role in the progression and development of systemic malignancies.
Journal of experimental therapeutics & oncology, 10(2), 163-164 (2013-01-29)
Adam B Shapiro
Biochemical pharmacology, 85(9), 1269-1277 (2013-02-19)
A novel, high-throughput-compatible assay for the ATP-dependent supercoiled DNA relaxing activity of human topoisomerase IIα (hTopoIIα) is described. The principle of detection is the preferential binding of the oligodeoxyribonucleotide BODIPY-TMR-5'-TTCTTCTTCT-3' to relaxed double-stranded plasmid containing the triplex forming sequence (TTC)9
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