Merck
CN
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文件

D141

Sigma-Aldrich

毛地黄皂苷

Used as non-ionic detergent

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别名:
Digitin
经验公式（希尔记法）:
C56H92O29
CAS号:
11024-24-1
分子量:
1229.31
Beilstein:
78654
EC 号:
234-255-6
MDL编号:
MFCD00077729
PubChem化学物质编号:
24893429
NACRES:
NA.25

生物来源

plant seeds (Digitalis purpurea)

质量水平

200

形式

powder

旋光性

[α]20/D −54°, c = 2.8 in methanol(lit.)

分子量

micellar avg mol wt 70,000

聚集数

60

杂质

<6.0% water

CMC

<0.5 mM (20-25°C)
<0.5 mM (20-25°C)

mp

230-240 °C (dec.) (lit.)

溶解性

H2O: ~5 % (w/v) (solubilized by heating to 95 °C - 98 °C and then cooling to room temp.)

SMILES字符串

C[C@@]12[C@]([C@@H]3C)([H])[C@](O[C@]34CC[C@@H](C)CO4)([H])[C@@H](O)[C@@]1([H])[C@@](CC[C@]5([H])[C@@]6(C[C@@H](O)[C@H](O[C@]([C@@H]([C@@H](O)[C@H]7O[C@@](O[C@H](CO)[C@@H](O)[C@@H]8O[C@@](OC[C@@H](O)[C@@H]9O)([H])[C@@H]9O)([H])[C@@H]8O[C@@](O[C@H](CO)[C@H

InChI

1S/C56H92O29/c1-19-7-10-56(75-17-19)20(2)31-45(85-56)37(67)32-22-6-5-21-11-26(24(61)12-55(21,4)23(22)8-9-54(31,32)3)76-50-42(72)39(69)44(30(16-60)80-50)81-53-48(47(36(66)29(15-59)79-53)83-49-40(70)33(63)25(62)18-74-49)84-52-43(73)46(35(65)28(14-58)78-52)82-51-41(71)38(68)34(64)27(13-57)77-51/h19-53,57-73H,5-18H2,1-4H3/t19-,20+,21+,22-,23+,24-,25-,26-,27-,28-,29-,30-,31+,32-,33+,34-,35+,36-,37+,38+,39-,40-,41-,42-,43-,44+,45-,46+,47+,48-,49+,50-,51+,52+,53+,54-,55+,56-/m1/s1

InChI key

UVYVLBIGDKGWPX-KUAJCENISA-N

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相关类别

一般描述

洋地黄皂苷是从紫色毛地黄植物（毛地黄）中提取的一种甾体皂苷，是一种多功能的非离子洗涤剂，在细胞生物学和生化研究中至关重要。其独特的属性使其成为探索细胞膜、膜蛋白和细胞内成分的宝贵工具。洋地黄皂苷′的关键特性和应用包括其能够有效溶解膜结合蛋白，同时保留其天然构象，使其成为研究膜蛋白相互作用和活性的理想工具。

此外，洋地黄皂苷选择性地透化富含胆固醇的质膜，并使细胞器膜保持完整。这一功能有利于特定细胞器的提取和纯化，有助于研究它们在细胞过程中的功能和作用。去垢剂′具有透化不同细胞类型的能力，可以通过将分子、抗体或酶引入细胞来研究细胞内成分和过程。此外，洋地黄皂苷还可用于免疫细胞化学实验，使得能够对细胞内蛋白质和结构进行标记和检测。

应用

洋地黄皂苷已与透化缓冲液一起用于细胞的环磷酸鸟苷-单磷酸腺苷（环GMP-AMP）刺激。
洋地黄皂苷已用于一项对从腹水肿瘤细胞中分离线粒体过程进行评估的研究中。 它还用于研究其对癌细胞中植物次生代谢物的细胞毒性的影响。

生化/生理作用

洋地黄皂苷在渗透细胞膜中起着关键作用。它能够裂解真核细胞，但不能裂解细菌膜。也可用于溶解细胞膜组分。它通过与细胞膜中的胆固醇相互作用形成孔隙。它因其温和的去污特性成为生物技术领域的首选。这种细胞膜活性化合物联合阿霉素治疗多药耐药癌细胞。

特点和优势

适用于细胞生物学和生化研究的高度通用化合物

质量

制备不包括不溶于水的成分。

其他说明

For additional information on our range of Biochemicals, please complete this form.

可比产品

产品编号
说明
价格

象形图

Skull and crossbonesHealth hazard
GHS06,GHS08

警示用语：

Danger

危险声明

H301 - H373

预防措施声明

P301 + P310 + P330 - P314

危险分类

Acute Tox. 3 Oral - STOT RE 2

储存分类代码

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges

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T1503
货号
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包装规格/数量

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索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the solubility of Product D141, Digitonin?

    The solubility of D141 is tested by heating a 5% solution in water to 95-98°C and then cooling the solution to room temperature. After cooling, the solution should remain practically clear for at least 24 hours. The product can also be dissolved at 100mg/4mL of warm ethanol.

  4. What is the solution stability of Product D141, Digitonin?

    We recommend making solutions fresh for use.

  5. How pure is Product D141, Digitonin?

    The purity of this product is evaluated by Thin Layer Chromatography. The actual results are lot specific and will be reported on the Certificate of Analysis of each lot.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

R W Moreadith et al.
Analytical biochemistry, 137(2), 360-367 (1984-03-01)
A new, improved procedure for isolating mitochondria from ascites tumor cells is described. The unique feature of this technique is the use of digitonin to make the cells susceptible to disruption by Teflon pestle/glass vessel homogenization. The yield and respiratory
Xianying A Cui et al.
Journal of visualized experiments : JoVE, (70)(70), e50066-e50066 (2012-12-29)
In eukaryotes, most of the messenger RNAs (mRNAs) that encode secreted and membrane proteins are localized to the surface of the endoplasmic reticulum (ER). However, the visualization of these mRNAs can be challenging. This is especially true when only a
Wattamon Srisakuldee et al.
Cardiovascular research, 103(1), 72-80 (2014-03-22)
Fibroblast growth factor 2 (FGF-2) protects the heart from ischaemia- and reperfusion-induced cell death by a mechanism linked to protein kinase C (PKC)ε-mediated connexin 43 (Cx43) phosphorylation. Cx43 localizes predominantly to gap junctions, but has also been detected at subsarcolemmal
Dominic S Alonzi et al.
Cellular and molecular life sciences : CMLS, 70(15), 2799-2814 (2013-03-19)
Endoplasmic reticulum-associated degradation (ERAD) is a key cellular process whereby misfolded proteins are removed from the endoplasmic reticulum (ER) for subsequent degradation by the ubiquitin/proteasome system. In the present work, analysis of the released, free oligosaccharides (FOS) derived from all
Xianying A Cui et al.
PLoS biology, 10(5), e1001336-e1001336 (2012-06-09)
In metazoans, the majority of mRNAs coding for secreted and membrane-bound proteins are translated on the surface of the endoplasmic reticulum (ER). Although the targeting of these transcripts to the surface of the ER can be mediated by the translation
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