D1821
DAF-FM
≥98% (HPLC)
别名:
4-氨基-5-甲基氨基-2′,7′-二荧光素, 二氨基荧光素-FM
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关于此项目
经验公式（希尔记法）:
C21H14F2N2O5
CAS Number:
分子量:
412.34
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.77
质量水平
方案
≥98% (HPLC)
表单
powder
储存条件
desiccated
颜色
white to brown
溶解性
DMSO: >1 mg/mL
运输
wet ice
储存温度
−20°C
SMILES字符串
CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(O)cc4Oc5cc(O)c(F)cc35
InChI
1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3
InChI key
DIJCILWNOLHJCG-UHFFFAOYSA-N
应用
DAF-FM已被用于小麦苗根部截面中一氧化氮（NO）的成像。它还被用于标记神经嵴（NC）细胞。
生化/生理作用
DAF-FM是一种高灵敏度、光稳定的荧光探针，可用于检测一氧化氮（NO）。
DAF-FM是一种高灵敏度、光稳定的荧光探针，可用于检测一氧化氮（NO）。DAF-2为初始化合物并且被广泛使用，但DAF-FM与DAF-2相比，对一氧化氮（NO）更敏感。DAF-FM比DAF-2具有更强的光稳定性以及更低的pH敏感性。DAF-FM不像二乙酸酯衍生物那样具有细胞渗透性，但它不需要被细胞溶质酶激活。
制备说明
产品从白色到棕色的颜色变化不会改变其性能。
免责声明
吸湿的
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Phillip Roder et al.
Scientific reports, 8(1), 5892-5892 (2018-04-14)
Local manipulation of complex tissues at the single-cell level is challenging and requires excellent sealing between the specimen and the micromanipulation device. Here, biological applications for a recently developed loading technique for a force- and pressure-controlled fluidic force microscope micropipette
Visualization of Nitric Oxide, Measurement of Nitrosothiols Content, Activity of NOS and NR in Wheat Seedlings
Adavi SB, et al.
Bio-protocol, 9(20) (2019)
Judy Choi et al.
Environmental health perspectives, 118(5), 589-595 (2010-05-05)
Silica nanoparticles (SiNPs) are being formulated for cellular imaging and for nonviral gene delivery in the central nervous system (CNS), but it is unclear what potential effects SiNPs can elicit once they enter the CNS. As the resident macrophages of
Nobuo Ueda et al.
Scientific reports, 6, 37546-37546 (2016-11-23)
In many marine invertebrates, larval metamorphosis is induced by environmental cues that activate sensory receptors and signalling pathways. Nitric oxide (NO) is a gaseous signalling molecule that regulates metamorphosis in diverse bilaterians. In most cases NO inhibits or represses this
Roberto Villalobos-Labra et al.
Placenta, 86, 35-44 (2019-07-28)
Pregestational maternal obesity (PGMO) associates with foetoplacental vascular endothelial dysfunction and higher risk for insulin resistance in the neonate. We characterised the PGMO consequences on the insulin response of the human foetoplacental vasculature. Umbilical veins were from pregnancies where the
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