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Merck
CN

D1946

Sigma-Aldrich

二氨基荧光素-FM 二乙酸酯

≥98% (HPLC), NO detector, film

别名:

4-氨基-5-（N-甲氨基）-3′,6′-双（乙酰氧基）-2′,7′-二氟-螺[ 异苯并呋喃-1 (3H) ,9′-[9H]黄原酮]-3-酮, DAF-FM DA

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关于此项目

经验公式（希尔记法）:
C25H18F2N2O7
CAS Number:
254109-22-3
分子量:
496.42
MDL编号:
MFCD16872050
UNSPSC代码:
12352200
PubChem化学物质编号:
329798717
NACRES:
NA.77

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Product Name

二氨基荧光素-FM 二乙酸酯, ≥98% (HPLC)

质量水平

100

方案

≥98% (HPLC)

表单

film

储存条件

desiccated

颜色

colorless to yellow

荧光

λex 500 nm; λem 515 nm in DMSO

运输

wet ice

储存温度

−20°C

SMILES字符串

CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(OC(C)=O)cc4Oc5cc(OC(C)=O)c(F)cc35

InChI

1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3

InChI key

BEVHTVRRVVEMEF-UHFFFAOYSA-N

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相关类别

应用

DAF-FM DA 已被用于测量肝组织匀浆中的一氧化氮 (NO) 水平。它还被用于检测人脐静脉内皮细胞 (HUVEC) 的 NO 染色。

生化/生理作用

DAF-FM DA 是一种用于检测一氧化氮 (NO) 的细胞渗透性荧光探针。
DAF-FM DA 是一种用于检测一氧化氮 (NO) 的细胞渗透性荧光探针。DAF-2 是最初的化合物，应用广泛，但 DAF-FM 和 DAF-FM DA 与 DAF-2 相比对一氧化氮 (NO) 更敏感，更耐光且pH 敏感性较低。DAF-FM DA 能很好地渗透到活细胞中，并通过胞浆酯酶迅速转化为水溶性 DAF-FM。

特点和优势

该化合物在受体分类和信号转导手册的一氧化氮合酶页面上有重点介绍。想要浏览手册的其他页面， 请单击此处。

免责声明

吸湿

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000357475
0000354599
0000354599
0000357475
0000073468

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Markus Schlicht et al.
Frontiers in plant science, 4, 351-351 (2013-09-24)
Powdery mildews are a diverse group of pathogenic fungi that can infect a large number of plant species, including many economically important crops. However, basic and applied research on these devastating diseases has been hampered by the obligate biotrophic lifestyle
Concentration-dependent osteogenic and angiogenic biological performances of calcium phosphate cement modified with copper ions
Zhang J, et al.
Materials Science and Engineering, C, 99, 1199-1212 (2019)
Longmei Zhai et al.
Plant physiology and biochemistry : PPB, 109, 515-524 (2016-11-12)
To cope with iron (Fe) deficiency, plants have evolved a wide range of adaptive responses from changes in morphology to altered physiological responses. Recent studies have demonstrated that nitric oxide (NO) is involved in the Fe-deficiency response through hormonal signaling
Bing Fang Luo et al.
Journal of experimental botany, 63(8), 3127-3136 (2012-03-02)
Nitrogen (N) management is a promising agronomic strategy to minimize cadmium (Cd) contamination in crops. However, it is unclear how N affects Cd uptake by plants. Wild-type and iron uptake-inefficient tomato (Solanum lycopersicum) mutant (T3238fer) plants were grown in pH-buffered
Shaoting Du et al.
Journal of experimental botany, 67(3), 893-904 (2015-11-27)
CO2 elevation often alters the plant's nitrate reductase (NR) activity, the first enzyme acting in the nitrate assimilation pathway. However, the mechanism underlying this process remains unknown. The association between elevated CO2-induced alterations of NR activity and nitric oxide (NO)
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