一般描述
Diffinity RapidTip可有效去除dNTP、引物和引物二聚体，并同时为从100 bp至10 kb纯的DNA片段提供超过90％的回收率。该功能性移液器吸头包含PCR纯化所需的一切。通过专有的吸附技术，该吸头可对PCR组分具有不同的亲和力。所分配的溶液可产生能力够即用于如DNA测序、SNP分析和微阵列打印等下游应用的高质量纯化DNA。
应用
Diffinity RapidTip®已被用于PCR产物纯化。
特点和优势
- 单步
- 回收90%的高质量dsDNA
- 优化用于25 μL RXN
法律信息
RapidTip is a registered trademark of Diffinity Genomics, Inc.
Lisa A Santry et al.
Virus research, 175(1), 30-44 (2013-04-16)
Maedi-visna virus (MVV) and caprine arthritis encephalitis virus (CAEV) are related members of a group of small ruminant lentiviruses (SRLVs) that infect sheep and goats. SRLVs are endemic in many countries, including Canada. However, very little is known about the
C N Roterman et al.
Proceedings. Biological sciences, 280(1764), 20130718-20130718 (2013-06-21)
The phylogeny of the superfamily Chirostyloidea (Decapoda: Anomura) has been poorly understood owing to limited taxon sampling and discordance between different genes. We present a nine-gene dataset across 15 chirostyloids, including all known yeti crabs (Kiwaidae), to improve the resolution
Myron T La Duc et al.
Applied and environmental microbiology, 78(16), 5912-5922 (2012-06-26)
Spacecraft hardware and assembly cleanroom surfaces (233 m(2) in total) were sampled, total genomic DNA was extracted, hypervariable regions of the 16S rRNA gene (bacteria and archaea) and ribosomal internal transcribed spacer (ITS) region (fungi) were subjected to 454 tag-encoded
Farha Masood et al.
Journal of materials science. Materials in medicine, 24(8), 1927-1937 (2013-05-16)
The synthesis of microbial polyhydroxyalkanoate is investigated in this work for it potential application as drug carrier for cancer therapy. The bacterial isolate Bacillus cereus FB11 has synthesized poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer under nutrient stress conditions using glucose as a sole carbon
Jason W Sahl et al.
Applied and environmental microbiology, 78(14), 4884-4892 (2012-05-15)
The sequencing and analysis of multiple housekeeping genes has been routinely used to phylogenetically compare closely related bacterial isolates. Recent studies using whole-genome alignment (WGA) and phylogenetics from >100 Escherichia coli genomes has demonstrated that tree topologies from WGA and
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