D2001
脱氧核糖核酸 钠盐 来源于大肠杆菌 菌株 B
Type VIII
别名:
DNA
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关于此项目
化学文摘社编号:
UNSPSC Code:
41106305
eCl@ss:
32160414
EC Number:
297-226-7
NACRES:
NA.51
MDL number:
Form:
fibers
Solubility:
water: 0.98-1.02 mg/mL, faintly hazy to hazy, colorless to light yellow
Storage temp.:
2-8°C
type
Type VIII
form
fibers
impurities
<2% protein
Quality Level
color
, white to white with yellow cast
solubility
water: 0.98-1.02 mg/mL, faintly hazy to hazy, colorless to light yellow
λmax
256-264 nm (15mM NaCl+1.5mM Trisodium Citrate PH7)
storage temp.
2-8°C
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Application
大肠杆菌DNA在研究中具有多种用途。 大肠杆菌DNA（ecDNA）可用于研究能够调节DNA结构和功能的DNA结合剂。 大肠杆菌DNA可用于溶液中DNA行为的物理化学研究，并评估物种特异性DNA的结构和结合结构域。该DNA对于基因组分析，包括PCR、噬菌体λ中的文库构建以及异源预杂交和杂交实验方案特别有用。
来自大肠杆菌B菌株的脱氧核糖核酸钠盐已被用于测量解链温度（Tm）和差异扫描量热法（DSC）研究中。它也已被用作聚合酶链反应（PCR）中的模板序列。
Preparation Note
按照Marmur, J., J. Mol. Biol., 3, 208 (1961)中的过程制备而得。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Joanna Will et al.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 13(3), 421-434 (2007-12-25)
Combined multidimensional liquid chromatography and electrospray ionisation tandem mass spectrometry was employed to analyse platinated tryptic peptides from Escherichia coli cells treated with the anticancer drug cis-[PtCl2(NH3)2] at pH 7.0. Prerequisites for the LC/LC/MS/MS analysis of protein targets that are
O Schröder et al.
Biochemical and biophysical research communications, 282(1), 219-227 (2001-03-27)
The DNA-binding protein H-NS compacts DNA and acts as a specific transcription factor regulating the expression of various bacterial genes. The small abundant protein binds to curved DNA without apparent sequence specificity and the exact nature of its DNA interaction
Solid-state magic-angle spinning NMR of outer-membrane protein G from Escherichia coli
Hiller M, et al.
Chembiochem, 6(9), 1679-1684 (2005)
R Richins et al.
Biotechnology progress, 17(2), 252-257 (2001-04-21)
The Escherichia coli DNA binding protein FIS is a transcriptional modulator involved in the regulation of many cellular processes, including the activation of rRNA synthesis. High-level overproduction of FIS in early, mid, or late log cultures resulted in growth-phase- and
Mitsumasa Hashimoto et al.
The Journal of biological chemistry, 278(31), 28501-28507 (2003-05-16)
Closely opposed lesions form a unique class of DNA damage that is generated by ionizing radiation. Improper repair of closely opposed lesions could lead to the formation of double strand breaks that can result in increased lethality and mutagenesis. In
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