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Merck
CN

D2001

Sigma-Aldrich

脱氧核糖核酸 钠盐 来源于大肠杆菌 菌株 B

Type VIII

别名:

DNA

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关于此项目

CAS Number:
EC 号:
MDL编号:
UNSPSC代码:
41106305
eCl@ss:
32160414
NACRES:
NA.51
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类型

Type VIII

质量水平

表单

fibers

杂质

<2% protein

储存温度

2-8°C

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应用

大肠杆菌DNA在研究中具有多种用途。 大肠杆菌DNA(ecDNA)可用于研究能够调节DNA结构和功能的DNA结合剂。 大肠杆菌DNA可用于溶液中DNA行为的物理化学研究,并评估物种特异性DNA的结构和结合结构域。该DNA对于基因组分析,包括PCR、噬菌体λ中的文库构建以及异源预杂交和杂交实验方案特别有用。
来自大肠杆菌B菌株的脱氧核糖核酸钠盐已被用于测量解链温度(Tm)和差异扫描量热法(DSC)研究中。它也已被用作聚合酶链反应(PCR)中的模板序列。

制备说明

按照Marmur, J., J. Mol. Biol., 3, 208 (1961)中的过程制备而得。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Corbin J England et al.
Analytical biochemistry, 616, 114099-114099 (2021-01-04)
Precipitation of DNA is performed frequently in molecular biology laboratories for the purpose of purification and concentration of samples and also for transfer of DNA into cells. Metal ions are used to facilitate these processes, though their precise functions are
William G Dundon et al.
International journal of medical microbiology : IJMM, 291(6-7), 545-550 (2002-03-14)
Infection of the stomach mucosa by the gastric pathogen Helicobacter pylori is accompanied by a large infiltration of neutrophils and monocytes which are believed to contribute substantially to H. pylori-induced gastritis. A protein was identified (HP-NAP for neutrophil-activating protein from
Geoffrey S Briggs et al.
The Journal of biological chemistry, 282(17), 12353-12357 (2007-02-20)
The DNA-binding protein, RdgC, is associated with recombination and replication fork repair in Escherichia coli and with the virulence-associated, pilin antigenic variation mediated by RecA and other recombination proteins in Neisseria species. We solved the structure of the E. coli
R Richins et al.
Biotechnology progress, 17(2), 252-257 (2001-04-21)
The Escherichia coli DNA binding protein FIS is a transcriptional modulator involved in the regulation of many cellular processes, including the activation of rRNA synthesis. High-level overproduction of FIS in early, mid, or late log cultures resulted in growth-phase- and
Mitsumasa Hashimoto et al.
The Journal of biological chemistry, 278(31), 28501-28507 (2003-05-16)
Closely opposed lesions form a unique class of DNA damage that is generated by ionizing radiation. Improper repair of closely opposed lesions could lead to the formation of double strand breaks that can result in increased lethality and mutagenesis. In

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