D2196
DAF-FM solution
5 mM in DMSO, ≥97% (HPLC)
别名:
4-Amino-5-methylamino- 2′,7′-difluorofluorescein, Diaminofluorescein-FM
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关于此项目
经验公式（希尔记法）:
C21H14F2N2O5
化学文摘社编号:
分子量:
412.34
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.77
Assay:
≥97% (HPLC)
Form:
DMSO solution
Storage condition:
desiccated
Quality Segment
assay
≥97% (HPLC)
form
DMSO solution
storage condition
desiccated
color
colorless to yellow, including pink
shipped in
wet ice
storage temp.
−20°C
SMILES string
Fc1cc2c(cc1O)Oc3c(cc(c(c3)O)F)C42OC(=O)c5c4ccc(c5N)NC
InChI
1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3
InChI key
DIJCILWNOLHJCG-UHFFFAOYSA-N
Application
DAF-FM solution has been used as a fluorescent probe for detecting nitric oxide (NO) from plant samples.
Biochem/physiol Actions
DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO).
DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM (Ex: 500 nm; Em: 515 nm; dectection limit: 3 nM) is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, but it does not need to be activated by cytosolic enzymes.
Features and Benefits
This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable