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D2196

DAF-FM solution

Name: DAF-FM solution
Brand: SIGMA

5 mM in DMSO, ≥97% (HPLC)

别名:

4-Amino-5-methylamino- 2′,7′-difluorofluorescein, Diaminofluorescein-FM

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关于此项目

经验公式（希尔记法）:

C₂₁H₁₄F₂N₂O₅

化学文摘社编号:

254109-20-1

分子量:

412.34

MDL number:

MFCD29073049

UNSPSC Code:

12352200

NACRES:

NA.77

Assay:

≥97% (HPLC)

Form:

DMSO solution

Storage condition:

desiccated

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属性

Quality Segment

100

assay

≥97% (HPLC)

form

DMSO solution

storage condition

desiccated

color

colorless to yellow, including pink

shipped in

wet ice

storage temp.

−20°C

SMILES string

Fc1cc2c(cc1O)Oc3c(cc(c(c3)O)F)C42OC(=O)c5c4ccc(c5N)NC

InChI

1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3

InChI key

DIJCILWNOLHJCG-UHFFFAOYSA-N

说明

Application

DAF-FM solution has been used as a fluorescent probe for detecting nitric oxide (NO) from plant samples.

Biochem/physiol Actions

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO).

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM (Ex: 500 nm; Em: 515 nm; dectection limit: 3 nM) is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, but it does not need to be activated by cytosolic enzymes.

Features and Benefits

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.