推荐产品
质量水平
形式
solution
技术
Southern blotting: suitable
异质活性
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
储存温度
−20°C
一般描述
Denhardt溶液是一种封闭剂,常规用于减少检测试剂与Northern和Southern印迹膜的非特异性结合。 这种50x浓缩液可以稀释到任何浓度,用于多种预杂交和杂交缓冲液。
组分
Denhardt溶液是1%牛血清白蛋白(BSA)、1%Ficoll和1%聚乙烯吡咯烷酮(PVP)的水溶液。
相关产品
产品编号
说明
价格
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
含少量动物源组分生物产品
Galanin receptor 3--a potential target for acute pancreatitis therapy.
Neurogastroenterology and Motility, 23(3), 23(3)-23(3) (2011)
Investigative ophthalmology & visual science, 41(7), 1629-1638 (2000-06-14)
The administration of glucocorticoids as topical or systemic medications may lead to the development of ocular hypertension through the induction of morphologic and biochemical changes in the trabecular meshwork leading to a reduction in the facility of aqueous outflow. Glucocorticoids
Journal of cell science, 103 ( Pt 3), 765-771 (1992-11-01)
The extracellular matrix glycoprotein, tenascin, is associated in vivo with mesenchyme undergoing osteogenesis and chondrogenesis, but is absent from mature bone and cartilage matrix. The expression of tenascin by osteoblastic cells in vitro has been investigated by immunoblotting and immunocytochemistry.
Nature protocols, 2(6), 1508-1514 (2007-06-16)
Here we describe a method for sensitive and specific histological detection of microRNAs (miRNAs) by in situ hybridization. The protocol focuses on the use of locked nucleic acids (LNAs), which are bi-cyclic RNA analogs that allow a significant increase in
Proceedings of the National Academy of Sciences of the United States of America, 89(18), 8542-8546 (1992-09-15)
The inhibitory effects of cyclosporin A (CsA) and FK506 on Fc epsilon receptor type I-initiated increases in cytokine mRNA and the expression of their intracellular binding proteins were studied in interleukin 3 (IL-3)-dependent, mouse bone marrow-derived mast cells (BMMCs). In
实验方案
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
Ficoll 400®是由蔗糖和环氧氯丙烷共聚而成的一种高度分支化的聚合物。Ficoll 400®是完全非离子性的。由于其含有丰富的羟基,Ficoll 400®具有很强的亲水性和水溶性。
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
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