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Merck
CN

D2532

Sigma-Aldrich

Denhardt溶液 50x

for Northern and Southern blotting, solution

别名:

blocking reagent, blocking solution, denhardts, hybridization buffer, hybridization solution, northern blocking solution, pre-hybridization, southern blocking solution

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关于此项目

UNSPSC代码:
12161700
NACRES:
NA.25

主要文件

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质量水平

300

表单

solution

技术

Southern blotting: suitable

异质活性

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

储存温度

−20°C

相关类别

一般描述

Denhardt溶液是一种封闭剂，常规用于减少检测试剂与Northern和Southern印迹膜的非特异性结合。 这种50x浓缩液可以稀释到任何浓度，用于多种预杂交和杂交缓冲液。

制备说明

Denhardt溶液是1%牛血清白蛋白（BSA）、1%Ficoll和1%聚乙烯吡咯烷酮（PVP）的水溶液。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

含少量动物源组分生物产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000411631
0000411631
0000375543
SLCN0620
0000320236

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实验方案

Ficoll® 400 for Gradient Centrifugation

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

用于梯度离心的Ficoll® 400

Ficoll 400®是由蔗糖和环氧氯丙烷共聚而成的一种高度分支化的聚合物。Ficoll 400®是完全非离子性的。由于其含有丰富的羟基，Ficoll 400®具有很强的亲水性和水溶性。

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern和Southern印迹实验方案及简介

Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

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