产品名称
SIGMAFAST™ 3,3′-二氨基联苯胺片, tablet, To prepare 1 mL
质量水平
表单
tablet
溶解性
deionized water: 1 mL, clear, pink
储存温度
−20°C
SMILES字符串
Nc1ccc(cc1N)-c2ccc(N)c(N)c2
InChI
1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2
InChI key
HSTOKWSFWGCZMH-UHFFFAOYSA-N
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应用
本品适于作为沉淀底物,在免疫组织和免疫印迹分析中原位检测过氧化物酶活性。DAB会生成高强度的耐酒精黑棕色色斑,是免疫组织分析的首选底物。经DAB染色后,玻片可进一步进行脱水和树脂封片处理,以便保存供后续比对。SIGMAFAST™ DAB片剂不需要另外的缓冲液或步骤来配制活性底物溶液。铝箔独立包装。
制备说明
将每片片剂溶于 1 mL 去离子水中,得到含 DAB 和过氧化氢尿素的即用型缓冲液。
法律信息
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
警示用语:
Danger
危险分类
Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Ox. Liq. 3 - Skin Irrit. 2 - STOT SE 3
靶器官
Central nervous system
储存分类代码
5.1B - Oxidizing hazardous materials
闪点(°F)
53.6 °F - closed cup
闪点(°C)
12 °C - closed cup
法规信息
危险化学品
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商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。
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