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Merck
CN

D4168

Sigma-Aldrich

SIGMAFAST 3,3′-二氨基联苯胺片

SIGMAFAST, tablet

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关于此项目

化学文摘社编号:
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.83
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产品名称

SIGMAFAST 3,3′-二氨基联苯胺片, tablet, To prepare 1 mL

质量水平

表单

tablet

溶解性

deionized water: 1 mL, clear, pink

储存温度

−20°C

SMILES字符串

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

InChI key

HSTOKWSFWGCZMH-UHFFFAOYSA-N

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应用

本品适于作为沉淀底物,在免疫组织和免疫印迹分析中原位检测过氧化物酶活性。DAB会生成高强度的耐酒精黑棕色色斑,是免疫组织分析的首选底物。经DAB染色后,玻片可进一步进行脱水和树脂封片处理,以便保存供后续比对。SIGMAFAST DAB片剂不需要另外的缓冲液或步骤来配制活性底物溶液。铝箔独立包装。

制备说明

将每片片剂溶于 1 mL 去离子水中,得到含 DAB 和过氧化氢尿素的即用型缓冲液。

法律信息

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

警示用语:

Danger

危险分类

Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Ox. Liq. 3 - Skin Irrit. 2 - STOT SE 3

靶器官

Central nervous system

储存分类代码

5.1B - Oxidizing hazardous materials

闪点(°F)

53.6 °F - closed cup

闪点(°C)

12 °C - closed cup

法规信息

危险化学品
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历史批次信息供参考:

分析证书(COA)

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商品

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

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