D4764
脱氧核糖核酸 来源于小牛胸腺
Genomic, unsheared
别名:
DNA 来源于小牛胸腺, 小牛胸腺DNA, 胸腺核酸
一般描述
从雄性和雌性小牛的胸腺分离的高质量双链模板DNA。
应用
小牛胸腺DNA非常适合用于作为DNA聚合酶测定的底物、长片段的扩增、以及作为沉淀时的载体DNA。
小牛胸腺DNA非常适合用作DNA聚合酶检测的底物、用于扩增非常长的片段以及用作DNA沉淀的载体。它被用作DNA荧光定量的标准品。它被用作参考物质，用于检测软骨组织消化样品的DNA含量。
生化/生理作用
42% GC；Tm = 87 °C.
％GC是指G/C碱基对的百分比。Tm是热变性曲线中点处的温度。
％GC是指G/C碱基对的百分比。Tm是热变性曲线中点处的温度。
特点和优势
•在37°C温育16小时后未检测到核酸酶活性
•高质量的模板DNA
•高质量的模板DNA
制备说明
将DNA溶解在分子生物级水中并轻轻摇动过夜，使终浓度为1mg/ml。
阴离子交换色谱纯化，用含1mM氯化钠、1mM EDTA和1mM Trizma HCl的溶液（pH 7.5）透析，并以约2 A260 单位/mL的浓度冻干。约20 260单位/mg DNA。
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves
法规信息
低风险生物材料
动植物源性产品
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Roel J H Custers et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 28(4), 429-435 (2009-11-04)
The purposes of the present study were to explore the surgical possibilities for replacement of the medial tibial plateau by a metallic implant in a large animal model and to examine the implications for the opposing cartilage. In six goats
A Châtel et al.
Ecotoxicology (London, England), 20(8), 1727-1740 (2011-06-10)
In the present study, we analyzed the effects of two major pollutants of the environment, tributyltin (TBT) and water-accommodated fraction (WAF) of diesel oil, on MAP kinase activation, apoptosis induction and DNA damage, in the marine sponge Suberites domuncula. Our
Nathalie W D Jansen et al.
Arthritis and rheumatism, 56(1), 199-207 (2006-12-30)
Joint bleeding, or hemarthrosis, leads in time to severe joint damage. This study was carried out to test the in vitro thresholds of exposure time and concentration that lead to irreversible joint damage, to add to the discussion on the
Sutha Shobana et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 107, 117-132 (2013-02-19)
Equilibrium studies of Ni(II), Cu(II) and Zn(II) mixed ligand complexes involving a primary ligand 5-fluorouracil (5-FU; A) and imidazoles viz., imidazole (him), benzimidazole (bim), histamine (hist) and L-histidine (his) as co-ligands(B) were carried out pH-metrically in aqueous medium at 310±0.1K
K Bishayee et al.
Cell proliferation, 46(2), 153-163 (2013-03-21)
Small aromatic compounds like flavonoids can intercalate with DNA molecules bringing about conformational changes leading to reduced replication and transcription. Here, we have examined one dietary flavonoid, quercetin (found in many fruit and vegetables), for possible anti-cancer effects, on HeLa
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