质量水平
表单
liquid
用途
sufficient for 250 reactions
特点
Long & Accurate PCR
dNTPs included: no
hotstart: no
浓度
1 unit/μL
技术
PCR: suitable
颜色
red
输入
purified DNA
运输
wet ice
储存温度
−20°C
一般描述
REDAccuTaq LA DNA polymerase allows for quick recognition in high throughput applications as well as direct loading of amplification products onto agarose gels for electrophoresis. The inert red dye has no effect on automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. However, the PCR product is easily separated from the dye by standard purification methods.
REDAccuTaq® long and accurate (LA) DNA Polymerase is a blend of Taq DNA polymerase and a small amount of thermostable proofreading polymerase that exhibits 3′→5′ exonuclease and an inert red dye that acts as a tracer and loading buffer. It is suitable for amplifying complex DNA templates like human genomic DNA.
应用
REDAccuTaq® LA DNA Polymerase produce long DNA amplicons for:
- Mutation analysis
- Cloning genes
- DNA sequencing
- cDNA library generation
- Amplicon sequencing
特点和优势
- Increased fidelity, up to 6.5× that of Taq DNA polymerase
- Efficiently and accurately produce amplicons up to 22 kb on genomic templates and up to 40 kb on less complex templates such as lambda or bacterial DNA
- Visual confirmation that enzyme has been added, and proper mixing has occurred
- No additional loading dyes needed. A post-reaction aliquot can be directly used on an agarose gel for electrophoresis
包装
The enzyme is provided with an optimized 10× reaction buffer for enhanced amplification of complex templates.
其他说明
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.
Store REDAccuTaq LA DNA Polymerase and AccuTaq LA 10x Buffer at –20 °C.
法律信息
本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
REDAccuTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves
法规信息
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商品
Long and accurate PCR applications address the needs for longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase.
实验方案
介绍RedTaq的应用和优势，包括用于基因组DNA PCR的标准RedTaq、热启动RedTaq和RedTaq。
Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.
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