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Merck
CN

D5042

Sigma-Aldrich

123 bp DNA分子量标准

for DNA electrophoresis

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别名:
DNA分子量标准, 用于 DNA 电泳的 DNA 梯状标志
UNSPSC代码:
41105335
NACRES:
NA.25

质量水平

100
200

形式

liquid

用途

100 uses (0.1 mg size)
250 uses (250 μg size)

适用性

suitable for electrophoresis (DNA)

储存温度

−20°C

应用

123 bp DNA 分子量标准已被用作随机扩增多态性 DNA-聚合酶链式反应 (PCR) 分析中的大小标记。它也被用作凝胶电泳的分子量标准。

组分

Sigma 的 123 bp DNA 分子量标准是在 10 mM Tris-HCl (pH 7.5)、50 mM NaCl 和 0.1 mM EDTA 溶液中提供的。

其他说明

包含35个片段,123-4182 bp
为实现最佳分辨率,建议采用的琼脂糖凝胶浓度为1.5%。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

分析证书(COA)

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Gene splicing and mutagenesis by PCR-driven overlap extension
Heckman KL and Pease LR
Nature Protocols, 2(4), 924-932 (2007)
Molecular characterization of lactic acid bacteria isolated from beef and stored using vacuum-packaging and advanced vacuum skin packaging systems
Inguane AS, et al.
CYTA-journal of Food (2011)
Straightforward procedure for laboratory production of DNA ladder
Lan VTT, et al.
Journal of Nucleic Acids, 2012 (2012)
Insulin-like growth factor I and II preserve myocardial structure in postinfarct swine
Kotlyar AA, et al.
British Heart Journal, 86(6), 693-700 (2001)
E J Gubbins et al.
The Journal of biological chemistry, 255(18), 8655-8662 (1980-09-25)
The organization and sequence of the rat preprolactin gene has been investigated. Analysis of two different plasmids containing pituitary cDNA inserts has provided the complete 681-nucleotide coding sequence of preprolactin as well as 17 nucleotides preceding the initiation codon and

商品

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

选择适合的标记物和分子量标准,用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。

相关内容

Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.

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