Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
pH
7.2-7.8
solubility
water: 9.6 g/L, clear
storage temp.
2-8°C
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General description
杜氏(Dulbecco′s)磷酸盐缓冲液(DPBS)是在组织培养中广泛使用的平衡盐溶液(BSS)。
Application
杜氏磷酸盐缓冲液已用于：
- 作为模拟生理溶液成分,体外研究Fe–20Mn–1.2C合金的降解行为
- 溶解朝鲜当归(Angelica gigas)粉末进行微囊化
- 在细胞培养物处理、愈伤试验、transwell试验和凋亡试验中用于清洗细胞
- 洗涤羊膜用于细胞分析
在制备和连续转移过程中，可使用D-PBS清洗细胞。
Biochem/physiol Actions
杜氏磷酸盐缓冲液可维持细胞渗透压平衡，作为灌洗、运输和稀释用液。它提供正常细胞代谢必需的水和大量无机离子。混入葡萄糖等糖后，这种缓冲盐可提供细胞代谢所需的主要能量。它的缓冲能力可使培养基维持在生理pH范围内。
Preparation Note
按照每升培养基9.6克粉末配制。
Other Notes
该D-PBS配方中没有Ca或Mg。也可提供加入Ca和Mg的配方
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| D5652-10L | 04061838259318 |
| D5652-200L | 04061834170662 |
| D5652-10X1L | 04061835514106 |
| D5652-1L | 04061837468315 |
| D5652-2000L | 04061837469190 |
| D5652-50L | 04061835518968 |
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