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D5758

焦碳酸二乙酯

96% (NT)

别名:

DEP, DEPC, 氧二甲酸二乙酯, 焦炭酸二乙酯, 重碳酸二乙酯

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关于此项目

线性分子式:
O(COOC2H5)2
化学文摘社编号:
1609-47-8
分子量:
162.14
UNSPSC Code:
12352202
NACRES:
NA.77
PubChem Substance ID:
24893964
EC Number:
216-542-8
Beilstein/REAXYS Number:
637031
MDL number:
MFCD00009106
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biological source

synthetic (microbial fermentation/ cell culture)

Quality Level

200

assay

96% (NT)

form

liquid

refractive index

n20/D 1.398 (lit.)

bp

93-94 °C/18 mmHg (lit.)

density

1.101 g/mL at 25 °C (lit.)

shipped in

wet ice

storage temp.

2-8°C

SMILES string

CCOC(=O)OC(=O)OCC

InChI

1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3

InChI key

FFYPMLJYZAEMQB-UHFFFAOYSA-N

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General description

焦碳酸二乙酯（DEPC）是一种用于灭活RNase酶并对水分和pH敏感的化学制品。在155℃时它在水溶液中可分解成乙醇和二氧化碳。DEPC对能将其分解成氨基甲酸乙酯这种潜在致癌物的氨也很敏感。

Application

焦碳酸二乙酯已在PCR反应中用于处理去离子水，以降低了RNA被RNase降解的风险。它还可以用于斑点印迹杂交，以稀释分离自不同微生物的总RNA。
蛋白中His及Tyr残基的修饰试剂。用于dsDNA结构破坏的稳健探针，可与完全或部分未堆叠的碱基发生反应。

Biochem/physiol Actions

DEPC是一种潜在的核酸酶抑制剂。0.1%的DEPC溶液用于灭活RNase。
它可以剂量和时间依赖的方式抑制兰尼碱与骨骼肌中兰尼碱/ Ca2+受体通道的结合，并提高SR囊泡的Ca2+通透性。
在溶液中浓度约为 0.1% (v/v) 时可使 RNase 失活，从而防止 RNA 被降解。

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H302

Hazard Classifications

Acute Tox. 4 Oral

存储类别

10 - Combustible liquids

flash_point_f

156.2 °F - closed cup

flash_point_c

69 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
WXBF7336V
WXBF6774V
WXBF5553V
WXBF4721V
WXBF3527V

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V Shoshan-Barmatz et al.
The Biochemical journal, 299 ( Pt 1), 177-181 (1994-04-01)
Exposure of junctional sarcoplasmic reticulum (SR) membranes or purified ryanodine receptor to the histidine-specific reagent diethyl pyrocarbonate (DEPC) led to concentration- and time-dependent inactivation of ryanodine binding. The pH-dependence of the inactivation of ryanodine binding by DEPC and the reversal
Properties of mouse globin messenger ribonucleic acid and its preparation in milligram quantities.
R Williamson et al.
Biochemistry, 10(16), 3014-3021 (1971-08-03)
H Perry-O'Keefe et al.
Journal of applied microbiology, 90(2), 180-189 (2001-02-13)
A method for rapid and simultaneous detection, identification and enumeration of specific micro-organisms using Peptide Nucleic Acid (PNA) probes is presented. The method is based on a membrane filtration technique. The membrane filter was incubated for a short period of
E C Williamson et al.
Journal of clinical microbiology, 39(1), 47-50 (2001-01-04)
Respiratory tract colonization with Scedosporium apiospermum in patients with chronic suppurative lung disease is a significant concern for lung transplantation candidates, since Scedosporium infections occurring posttransplantation are usually untreatable. Up to 10% of patients with cystic fibrosis attending our respiratory
A new method based on the use of diethyl pyrocarbonate as a nuclease inhibitor for the extraction of undegraded nucleic acid from plant tissues.
F Solymosy et al.
European journal of biochemistry, 5(4), 520-527 (1968-09-24)
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商品

Water for Nuclease-sensitive Molecular Biology Studies

Nuclease-free water (or PCR water) is needed in molecular biology to avoid sample degradation during PCR, sequencing, etc. Ultrafiltration provides a quicker, safer, and more sustainable alternative to DEPC treatment when preparing RNase-free and DNase-free water.

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全球贸易项目编号

货号GTIN
D5758-50ML04061838255211
D5758-100ML04061838255198
D5758-25ML04061838255204
D5758-5ML04061838087348

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