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Merck
CN

D6429

Sigma-Aldrich

DMEM - high glucose

With sodium bicarbonate, ʟ-glutamine and sodium pyruvate, sterile liquid, suitable for cell culture

别名:

DME, DMEM

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.71

product name

杜氏改良 Eagle 培养基 - 高葡萄糖, With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

质量水平

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

HEPES: no
NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high

运输

ambient

储存温度

2-8°C

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一般描述

这种DMEM高葡萄糖培养基是一种添加了丙酮酸钠的1x完全培养基。 它还与原始DMEM高葡萄糖配方的不同点在于用吡哆醇代替了吡哆醛。 吡哆醛是一种不稳定的培养基成分。

应用

Dulbecco′s Modified Eagle′s Medium - 高葡萄糖培养基已用于细胞培养。
Dulbecco′s Modified Eagle′s Medium(DMEM)是对Basal Medium Eagle (BME)的一种改良,含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此,它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养,以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外,葡萄糖浓度也被提高至4500 mg/L,因此被命名为“DMEM/High”。

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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