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Merck
CN

D6920

2′-脱氧腺苷5′-三磷酸 钠盐 溶液

10 mM

别名:

dATP

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关于此项目

EC 号:
UNSPSC代码:
41106305
NACRES:
NA.52
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方案

≥99%

表单

liquid

浓度

10 mM

颜色

colorless

异质活性

DNase, RNase, NICKase, none detected

运输

dry ice

储存温度

−20°C

SMILES字符串

[Na+].Nc1ncnc2n(cnc12)[C@H]3C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)([O-])=O)O3

InChI

1S/C10H16N5O12P3.Na/c11-9-8-10(13-3-12-9)15(4-14-8)7-1-5(16)6(25-7)2-24-29(20,21)27-30(22,23)26-28(17,18)19;/h3-7,16H,1-2H2,(H,20,21)(H,22,23)(H2,11,12,13)(H2,17,18,19);/q;+1/p-1/t5-,6+,7+;/m0./s1

InChI key

YJWCICGGRLOGEH-VWZUFWLJSA-M

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一般描述

2′-脱氧腺苷5′-三磷酸(dATP)由脱氧核糖上的一个碱基和糖上与一个由三个磷酸残基组成的链相结合的糖上的5′-羟基组成。细胞利用dATP通过DNA聚合酶合成DNA。

应用

2′-脱氧腺苷5′-三磷酸盐钠盐可用于DNA合成反应,如PCR、DNA测序和分子克隆技术。
2′-Deoxyadenosine 5′-triphosphate sodium salt solution has been used as a component of the dNTP mix for labelling of ribosomal 18s rDNA probe and telomeric (TTAGG)n probe.

储存分类代码

11 - Combustible Solids

WGK

WGK 3


历史批次信息供参考:

分析证书(COA)

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Kuznetsova V G, et al.
Protocols for Cytogenetic Mapping of Arthropod Genomes., 351-380 (2015)
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Critical reviews in biochemistry and molecular biology, 47(1), 50-63 (2011-11-05)
Ribonucleotide reductase (RNR) is the only source for de novo production of the four deoxyribonucleoside triphosphate (dNTP) building blocks needed for DNA synthesis and repair. It is crucial that these dNTP pools are carefully balanced, since mutation rates increase when
Kevin N Kirouac et al.
Nucleic acids research, 41(3), 2060-2071 (2012-12-27)
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Cell death & disease, 2, e197-e197 (2011-09-02)
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Nearly every enzyme undergoes a significant change in structure after binding it's substrate. Experimental and theoretical analyses of the role of changes in HIV reverse transcriptase structure in selecting a correct substrate are presented. Atomically detailed simulations using the Milestoning

实验方案

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法(<1min), and results in higher yields and more specificity over standard PCR methods.

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