D7290
脱氧核糖核酸 来源于鲱鱼精子
single-stranded for hybridization
别名:
DNA
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关于此项目
化学文摘社编号:
UNSPSC Code:
41106310
eCl@ss:
32160414
EC Number:
309-566-6
NACRES:
NA.52
MDL number:
产品名称
脱氧核糖核酸 来源于鲱鱼精子, single-stranded for hybridization
biological source
(herring sperm)
grade
Molecular Biology
form
solution
shipped in
dry ice
storage temp.
−20°C
Quality Level
Application
为了减少探针与底物的非特异性杂交，必须使用封闭剂。一般结合采用封闭剂、去污剂和变性、片段化 DNA来实现此目的。Sigma 提供来自多种物种的超声变性 DNA，在RNA印迹和 DNA印迹以及其他核酸杂交技术中用作封闭剂。
在核酸杂交中，有许多因素影响信噪比。这些因素包括溶剂（甲酰胺）的存在、杂交温度、杂交长度、杂交溶液的体积、搅拌的程度和方法、封闭试剂的使用、探针的浓度和比活度、使用分子制剂以提高核酸再结合率以及洗膜过程中使用的严格程度。
为了减少探针与底物的非特异性杂交，必须使用封闭剂。一般结合采用封闭剂、去污剂和变性、片段化 DNA来实现此目的。Sigma 提供来自多种物种的超声变性 DNA，在RNA印迹和 DNA印迹以及其他核酸杂交技术中用作封闭剂。
为了减少探针与底物的非特异性杂交，必须使用封闭剂。一般结合采用封闭剂、去污剂和变性、片段化 DNA来实现此目的。Sigma 提供来自多种物种的超声变性 DNA，在RNA印迹和 DNA印迹以及其他核酸杂交技术中用作封闭剂。
来自鲱鱼精子的脱氧核糖核酸适合用作Southern杂交的封闭剂。可用于制备 BrdU 免疫组化小鼠脑切片。也用于斑点印迹膜的杂交反应。
General description
适合在 Southern 杂交中用作封闭剂。
Other Notes
溶液中的 DNA 将在室温下静置后重新退火，因此建议将溶液煮沸 10 分钟，然后在使用前在冰上冷却至少 5 分钟。
Preparation Note
将该 DNA 进行酚-氯仿提取、乙醇沉淀和超声处理以产生与 587 和 831 碱基对标记片段相同的单链片段。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
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