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D8130

5,5′-二硫代双（2-硝基苯甲酸）

Name: 5,5′-二硫代双（2-硝基苯甲酸）
Brand: SIGMA

≥98%, BioReagent, suitable for determination of sulfhydryl groups

别名:

6,6′-二硝基-3,3′-二硫代苯甲酸, DTNB, Ellman 试剂, 二硫代硝基苯甲酸, 双(3-羧基-4-硝基苯基)二硫

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关于此项目

线性分子式:

[-SC₆H₃(NO₂)CO₂H]₂

化学文摘社编号:

69-78-3

分子量:

396.35

UNSPSC Code:

12352106

NACRES:

NA.26

PubChem Substance ID:

24894189

EC Number:

200-714-4

Beilstein/REAXYS Number:

1896821

MDL number:

MFCD00007140

Assay:

≥98%

Form:

powder

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product line

BioReagent

Quality Level

200

assay

≥98%

form

powder

mp

240-245 °C (dec.) (lit.)

suitability

suitable for determination of sulfhydryl groups

SMILES string

OC(=O)c1cc(SSc2ccc(c(c2)C(O)=O)[N+]([O-])=O)ccc1[N+]([O-])=O

InChI

1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)

InChI key

KIUMMUBSPKGMOY-UHFFFAOYSA-N

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General description

5,5′双二硫代(2-硝基苯甲酸)也称为DTNB或Ellman′s试剂。这是一种与巯基相互作用的水溶性试剂，在中性pH下方便地应用。硫代阴离子(RS^-)与Ellman′s试剂(DTNB^2-)反应生成2-硝基-5-硫代苯甲酸盐阴离子(TNB^2-)和一种混合二硫化物(R-S-TNB^-)。TNB^2- 具有较高的摩尔吸收系数，在412nm处有吸收。这种吸收与pH无关，在pH>7.3。

Application

Ellman′s试剂可用于乙酰胆碱酯酶的检测，并可对半胱氨酸残基进行特异性标记。该试剂是测定蛋白质、多肽和组织中游离巯基含量的敏感试剂。

存储类别

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Gene therapy delivering a paraoxonase 1 variant offers long-term prophylactic protection against nerve agents in mice.

Venkaiah Betapudi et al.

Science translational medicine, 12(527) (2020-01-24)

Chemical warfare nerve agents are organophosphorus chemical compounds that induce cholinergic crisis, leaving little or no time for medical intervention to prevent death. The current chemical treatment regimen may prevent death but does not prevent postexposure complications such as brain

Staphylococcal extracellular adherence protein induces platelet activation by stimulation of thiol isomerases.

Anne Bertling et al.

Arteriosclerosis, thrombosis, and vascular biology, 32(8), 1979-1990 (2012-04-28)

Staphylococcus aureus can induce platelet aggregation. The rapidity and degree of this correlates with the severity of disseminated intravascular coagulation, and depends on platelet peptidoglycans. Surface-located thiol isomerases play an important role in platelet activation. The staphylococcal extracellular adherence protein

Quick measurement of protein sulfhydryls with Ellman's reagent and with 4,4'-dithiodipyridine.

Christian K Riener et al.

Analytical and bioanalytical chemistry, 373(4-5), 266-276 (2002-07-12)

Since its introduction in 1959, Ellman's reagent (5,5'-dithio-bis(2-nitrobenzoic acid)) has been the favorite reagent for spectrophotometric measurement of protein sulfhydryls. Meanwhile however, evidence has accumulated that many protein sulfhydryls give an incomplete reaction with Ellman's reagent, even during prolonged assay

Reassessment of Ellman's reagent.

P W Riddles et al.

Methods in enzymology, 91, 49-60 (1983-01-01)

Ellman's reagent: 5,5'-dithiobis(2-nitrobenzoic acid)--a reexamination.

P W Riddles et al.

Analytical biochemistry, 94(1), 75-81 (1979-04-01)

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实验方案

Enzymatic Assay of Chloramphenicol Acetyltransferase

To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.

全球贸易项目编号

货号	GTIN
D8130-150MG	04061833412954
D8130-50MG	04061833690161
D8130-10G	04061835544141
D8130-4MG	04061833690154
D8130-100G	04061835544134
D8130-25G	04061835544165
D8130-1G	04061835544158
D8130-8MG	04061833460979
D8130-500MG	04061835544172
D8130-5G	04061835544189

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