D8187
JumpStart™ REDTaq® DNA Polymerase
Hot-start Taq enzyme with inert dye, 10X buffer included
别名:
Hot start DNA polymerase, Hot start Taq
质量水平
表单
liquid
用途
sufficient for 250 reactions
sufficient for 2500 reactions
sufficient for 50 reactions
特点
dNTPs included: no
hotstart
浓度
1 unit/μL
技术
PCR: suitable
颜色
red
输入
purified DNA
适用性
suitable for PCR
运输
wet ice
储存温度
−20°C
相关类别
一般描述
JumpStart™ REDTaq® DNA Polymerase is Sigma′s high performance Taq DNA Polymerase blended with JumpStart Taq antibody and an inert red dye tracer. Extensive testing with a variety of primers and templates indicates that the performance of JumpStart REDTaq DNA Polymerase is equivalent to, or better than, that of standard Taq polymerase.
Since the red tracer has no effect on the amplification process, a sample can be easily re-amplified such as in “nested PCR”. The presence of the dye also has no effect on automated DNA sequencing; ligase mediated ligations, exonucleolytic PCR product digestion, and transformation. Though exceptions may exist, the dye is generally inert in restriction enzyme digestions. If necessary, the dye can be removed from the amplicon by routine purification methodologies.
Since the red tracer has no effect on the amplification process, a sample can be easily re-amplified such as in “nested PCR”. The presence of the dye also has no effect on automated DNA sequencing; ligase mediated ligations, exonucleolytic PCR product digestion, and transformation. Though exceptions may exist, the dye is generally inert in restriction enzyme digestions. If necessary, the dye can be removed from the amplicon by routine purification methodologies.
应用
JumpStart™ REDTaq® DNA Polymerase has been used in polymerase chain reaction (PCR) for the amplification of:
- insulin-enterotoxin ricin fusion gene (INS-RTB)
- endothelial cells DNA derived from reverse transcribed RNA
- leg genomic DNA from cricket flies
- mitochondrial gene by conventional PCR
特点和优势
- Reduces non-specific amplification
- Increased target yield and specificity
- Higher the amplification irrespective of the target concentration
- Reduce set-up time and eliminate concerns associated with manual or wax hot start methods
- Visual confirmation that the enzyme has been added and that proper component mixing of the reaction has occurred
- Samples can be loaded directly onto an agarose gel for electrophoresis without loading buffers or tracking dyes
- Assembled PCR reactions can be placed at room temperature for up to 2 hours
包装
The enzyme is provided with an optimized 10× reaction buffer.
其他说明
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.
View more detailed information on JumpStart REDTaq enzymes at www.sigma-aldrich.com/hotstart.
法律信息
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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