质量水平
表单
liquid
用途
sufficient for 1000 reactions
sufficient for 250 reactions
sufficient for 2500 reactions
特点
Difficult Templates/Specialty Enzymes PCR
dNTPs included: no
hotstart: no
浓度
1 unit/μL
技术
PCR: suitable
颜色
red
输入
purified DNA
应用
agriculture
运输
wet ice
储存温度
−20°C
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一般描述
DNA聚合酶是通过将核苷酸（DNA 组成部分）组装成DNA分子的酶。这些酶对于DNA复制必不可少，通常成对工作。它从单个原始DNA分子产生两条相同的DNA链。在此过程中，DNA聚合酶“读取”现有的DNA链，以构建两条与现有的DNA链匹配的新链。
REDTaq®基因组DNA聚合酶是Taq DNA聚合酶与惰性红色染料的独特混合物。这种特殊的配方旨在增强更复杂或基因组模板的扩增。REDTaq基因组DNA聚合酶高度灵敏，可提高良率并延长产品长度。它具有REDTaq DNA聚合酶的所有优点，如易于观察加酶和完全反应混合，以及直接加载到琼脂糖凝胶中。这种惰性红色染料不会影响自动或手动测序、限制消化或其他下游应用。这种染料可以用任何标准的提纯方法轻易去除。
应用
REDTaq®基因组DNA聚合酶用于从基因组DNA进行常规PCR扩增。
特点和优势
- 增强基因组和复杂 DNA 模板扩增
- 在实现与 Taq DNA 聚合酶相同性能的同时，具有适用于高通量应用的更方便形式
- 快速识别并确认适当混合
- 无需上样缓冲液或跟踪染料。样品可直接从反应中提取并上样至琼脂糖凝胶上
- 样品可以像嵌套PCR一样进行重复扩增
- 红色染料的迁移速度比溴酚蓝快
- 由于适当的混合，反应一致性更佳
其他说明
在74°C下，一个单位可在30分钟内将10 nM总dNTP掺入酸可沉淀的DNA中。
法律信息
本产品的使用涵盖在以下一项或多项美国专利以及美国以外的相应专利要求中：美国 8,404,464 和美国7,972,828。本产品的采购包括上述专利要求下的有限、不可转让的诉讼豁免。
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
含少量动物源组分生物产品
常规特殊物品
此项目有
L M Winton et al.
Phytopathology, 92(1), 112-116 (2008-10-24)
ABSTRACT Phaeocryptopus gaeumannii is a widespread foliar parasite of Douglas-fir. Although normally innocuous, the fungus also causes the defoliating disease Swiss needle cast in heavily infected needles. The extent of P. gaeumannii colonization in Douglas-fir foliage was estimated with real-time
J A F Demandt et al.
Scientific reports, 11(1), 425-425 (2021-01-13)
Hypoxia is prevalent in atherosclerotic plaques, promoting plaque aggravation and subsequent cardiovascular disease (CVD). Transmembrane protein carbonic anhydrase IX (CAIX) is hypoxia-induced and can be shed into the circulation as soluble CAIX (sCAIX). As plaque macrophages are hypoxic, we hypothesized a
Gregory G Martin et al.
The Journal of biological chemistry, 278(24), 21429-21438 (2003-04-03)
Although liver fatty acid-binding protein (L-FABP) is an important binding site for various hydrophobic ligands in hepatocytes, its in vivo significance is not understood. We have therefore created L-FABP null mice and report here their initial analysis, focusing on the
Jill A Fahrner et al.
Cancer research, 62(24), 7213-7218 (2002-12-25)
We examined the relationship between aberrant DNA hypermethylation and key histone code components at a hypermethylated, silenced tumor suppressor gene promoter in human cancer. In lower eukaryotes, methylated H3-lysine 9 (methyl-H3-K9) determines DNA methylation and correlates with repressed gene transcription.
Preparation of genomic DNA from Dictyostelium discoideum for PCR analysis.
Steve J Charette et al.
BioTechniques, 36(4), 574-575 (2004-04-20)
实验方案
介绍RedTaq的应用和优势，包括用于基因组DNA PCR的标准RedTaq、热启动RedTaq和RedTaq。
Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.
Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.
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