biological source
bovine articular cartilage
form
salt-free, lyophilized powder
mol wt
~100 kDa
packaging
glass bottle of 0.5 mg
technique(s)
cell culture | mammalian: suitable
solubility
PBS: ≥2.00 mg/mL, clear, colorless to yellow
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
cow ... DCN(280760)
General description
核心蛋白聚糖是一种小蛋白聚糖,由附着在40 kDa核心蛋白上的软骨素/硫酸皮肤素粘多糖组成。 它主要存在于关节软骨的细胞外基质中。 核心蛋白聚糖通过其核心蛋白与I型和II型胶原蛋白相互作用,从而延迟原纤维生成。 核心蛋白聚糖还与纤连蛋白、血小板反应蛋白、TGF-β亚型和EGF受体相互作用。
牛关节软骨核心蛋白聚糖是一种蛋白聚糖,属于小富含亮氨酸重复序列蛋白聚糖和蛋白质(SLRP)家族。核心蛋白聚糖几乎存在于所有组织中。它有一个由富含亮氨酸重复序列(LRR)组成的蛋白核心,两侧是两个富含半胱氨酸的区域。
Application
牛关节软骨核心蛋白聚糖可用于固相结合试验(ELISA)、结合试验、结合抑制试验、寄生虫结合试验、支架制备、石英晶体微天平试验、CSA(硫酸软骨素A)结合抑制试验以及聚糖和聚糖生物素化。
Biochem/physiol Actions
核心蛋白聚糖在引导原纤维生成过程中胶原蛋白的正确组装中发挥重要作用。核心蛋白聚糖的缺乏会导致组织结构和功能的改变它可以控制EGF和TGF-β等生长因子。核心蛋白聚糖可在聚合化I型胶原蛋白的无细胞构建物中阻止原纤维聚集成纤维和原纤维生成。
Other Notes
核心蛋白聚糖是一种约100kDa的蛋白聚糖,由一个40kDa的核心蛋白和一个软骨素或硫酸皮肤素粘多糖链组成。来自冻干前的0.2 μm过滤溶液。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
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有五种已发现的糖胺聚糖链(参见图1)透明质酸不是硫酸化的,但其他糖胺聚糖链在链的不同位置含有硫酸根取代基。
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