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D8537

Sigma-Aldrich

杜氏磷酸盐缓冲盐水

Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

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别名:
DPBS
MDL编号:
MFCD00131855
eCl@ss:
32129211
NACRES:
NA.75

质量水平

300

无菌性

sterile-filtered

形式

liquid

technique(s)

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

glucose: no (Dextro)
sodium pyruvate: no

运输

ambient

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相关类别

一般描述

杜氏磷酸盐缓冲盐水（D-PBS）是一种用于处理和培养哺乳动物细胞的平衡盐溶液（BSS）。DPBS缓冲液可用于冲洗、洗涤并稀释哺乳动物细胞，同时维持渗透平衡。它提供正常细胞代谢所必需的水和无机离子。DPBS的钙和镁有助于细胞的结合和成团。不含这些离子的DPBS可用于悬浮后细胞的洗涤和润洗。磷酸缓冲体系将pH维持在生理性范围内。

应用

Dulbecco′s磷酸盐缓冲液已用于：

  • 稀释新鲜红细胞用于研究红细胞聚集物的捕获。
  • 清洗微量滴定板的微孔
  • 在裂解缓冲液中重悬细胞以前清洗细胞进行DNA分离
  • 作为胚胎保存培养基的成分用于胚胎的玻璃化。

其他说明

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产品编号
说明
价格

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

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包装规格/数量

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索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the formulation for Product D8537, Dulbecco's Phosphate?

    The formulation for 1 liter is 8 g Sodium Chloride, 0.2 g Potassium Phosphate, monobasic, 1.15 g Sodium Phosphate, dibasic, and 0.2 g Potassium Chloride.    

  4. Is Product D8537, Dulbecco's Phosphate, a 1X or 10 X concentration?

    This is a 1X concentration. The 10X concentration is listed under product D1408.

  5. What is the pH of a solution of Product D8537, Dulbecco's Phosphate?

    The pH will be in the range of 7.1-7.5, according to the specification sheet.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. What is a Salt Toxicity Test?

    The Cell Culture assay is called a Salt Toxicity Test (previously called Cell Culture Salt Testing). A more complete description is below:Cells are harvested and counted.  They are resuspended in DPBS and incubated at 37°C, 5% CO2 for 1 hour.  The cells are counted and %viability determined.  The cells are then cultured for an additional 7 hours (total of 8 hours).  The cells are counted again and the % viability determined.  The viability at 1 hr has to be >95% of the original viability.  The viability at 8 hrs must be >75% of the original viability.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Monitoring of bacteria concentrations is of great importance in drinking water management. Continuous real-time monitoring enables better microbiological control of the water and helps prevent contaminated water from reaching the households. We have developed a microfluidic sensor with the potential
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