Merck
CN

D8899

Sigma-Aldrich

脱氧核糖核酸,单链 来源于小牛胸腺

lyophilized powder

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别名:
DNA 单链
CAS号:
EC 号:
MDL编号:
eCl@ss:
32160414
NACRES:
NA.52

等级

for molecular biology

质量水平

形式

lyophilized powder

分子量

~50 kb

组成

ssDNA, ≥65%

储存温度

−20°C

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此商品
D4522D4764D8273
form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

~50 kb

mol wt

-

mol wt

>20 kb

mol wt

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

-

Quality Level

-

composition

ssDNA, ≥65%

composition

-

composition

-

composition

-

一般描述

从雄性和雌性小牛的胸腺分离的高质量单链模板 DNA。

应用

小牛胸腺 DNA 非常适合用于作为 DNA 聚合酶测定的底物、超长片段扩增,以及作为沉淀时的载体 DNA。在 DNA 荧光测定和 DNA 结合试验中,它可用作测定标准品。
小牛胸腺DNA非常适合用于作为 DNA 聚合酶测定的底物、长片段的扩增、以及作为沉淀时的载体 DNA。

特点和优势

•已激活
•在 37 °C,温育 16 小时后,未检测到核酸酶活性
•高质量模板 DNA

数量

1mg DNA 约为 25 个 A260 单位。

制备说明

以小牛胸腺 DNA 为原料,根据改良的 Alberts 和 Herrick 方法进行制备。

重悬

将 DNA 在分子生物级水中溶解过夜,终浓度为 1mg/ml。

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves


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J A Hansen et al.
Journal of animal science, 75(7), 1810-1821 (1997-07-01)
We evaluated the effects of recombinant porcine somatotropin (pST) and the beta-adrenergic agonist salbutamol on plasam endocrine and metabolite profiles and muscle chemistry in three genotypes of growing barrows (n = 96, 139 d old). Treatments were in a 2
H Kamei
Cell biology international, 20(11), 769-775 (1996-11-01)
The anti-desmin monoclonal antibodies (MAbs) DSB389, AC54, and DSB860 recognize intermediate filaments (IFs) and nuclear antigens that appear granular, locate around chromosomes, and are insoluble following 0.5% Triton X-100 and cetyltrimethylammonium bromide (CTAB) extraction. Nuclear antigens of the MAbs were
José Luis García-Giménez et al.
Journal of inorganic biochemistry, 121, 167-178 (2013-02-07)
Ternary copper(II) complexes [Cu(NST)2(phen)] (1) and [Cu(NST)2(NH3)2]·H2O (2) [HNST=N-(4,5-dimethylthiazol-2-yl)naphthalene-1-sulfonamide] were prepared and characterized by physico-chemical techniques. Both 1 and 2 were structurally characterized by X-ray crystallography. The crystal structures show the presence of a distorted square planar CuN4 geometry in
Inessa Halets et al.
International journal of pharmaceutics, 454(1), 1-3 (2013-07-09)
In most articles, cytotoxicity of cationic polyamidoamine (PAMAM) dendrimers toward red blood cells has been exclusively explained by their surface charge. We have focused on dendrimer hydrophobicity as a second possible factor that determines this cytotoxicity. Using PAMAM-NH2 dendrimers from
Qiong Guo et al.
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A divanadium(V) complex, [V2O3(o-van-val)2] (o-van-val=Schiff base derived from o-vanillin and L-valine), has been synthesized and structurally characterized. The crystal structure shows that both of the vanadium centers in the complex have a distorted octahedral coordination environment composed of tridentate Schiff

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