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Merck
CN

D8899

脱氧核糖核酸,单链 来源于小牛胸腺

lyophilized powder

别名:

DNA 单链

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关于此项目

化学文摘社编号:
UNSPSC Code:
41106310
eCl@ss:
32160414
EC Number:
293-507-3
NACRES:
NA.52
MDL number:
Biological source:
bovine (calf) thymus
Form:
lyophilized powder
Storage temp.:
−20°C
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biological source

bovine (calf) thymus

Quality Level

grade

Molecular Biology

form

lyophilized powder

mol wt

~50 kb

composition

ssDNA, ≥65%

storage temp.

−20°C

General description

从雄性和雌性小牛的胸腺分离的高质量单链模板 DNA。

Application

小牛胸腺 DNA 非常适合用于作为 DNA 聚合酶测定的底物、超长片段扩增,以及作为沉淀时的载体 DNA。在 DNA 荧光测定和 DNA 结合试验中,它可用作测定标准品。
小牛胸腺DNA非常适合用于作为 DNA 聚合酶测定的底物、长片段的扩增、以及作为沉淀时的载体 DNA。

Features and Benefits

•已激活
•在 37 °C,温育 16 小时后,未检测到核酸酶活性
•高质量模板 DNA

Preparation Note

以小牛胸腺 DNA 为原料,根据改良的 Alberts 和 Herrick 方法进行制备。
1mg DNA 约为 25 个 A260 单位。
将 DNA 在分子生物级水中溶解过夜,终浓度为 1mg/ml。

存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

法规信息

低风险生物材料

此项目有


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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J A Hansen et al.
Journal of animal science, 75(7), 1810-1821 (1997-07-01)
We evaluated the effects of recombinant porcine somatotropin (pST) and the beta-adrenergic agonist salbutamol on plasam endocrine and metabolite profiles and muscle chemistry in three genotypes of growing barrows (n = 96, 139 d old). Treatments were in a 2
H Kamei
Cell biology international, 20(11), 769-775 (1996-11-01)
The anti-desmin monoclonal antibodies (MAbs) DSB389, AC54, and DSB860 recognize intermediate filaments (IFs) and nuclear antigens that appear granular, locate around chromosomes, and are insoluble following 0.5% Triton X-100 and cetyltrimethylammonium bromide (CTAB) extraction. Nuclear antigens of the MAbs were
Qiong Guo et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 106, 155-162 (2013-02-05)
A divanadium(V) complex, [V2O3(o-van-val)2] (o-van-val=Schiff base derived from o-vanillin and L-valine), has been synthesized and structurally characterized. The crystal structure shows that both of the vanadium centers in the complex have a distorted octahedral coordination environment composed of tridentate Schiff
Hanping He et al.
Biosensors & bioelectronics, 49, 282-289 (2013-06-19)
We have developed a simple and label-free electrochemical assay to detect CGG trinucleotide repeat. For this purpose, a new bifunctional probe (FecNCD2) was developed, in which a recognition part (naphthyridine carbamate dimmer, NCD) was connected with an electro-active part (ferrocenyl
DNA-cellulose chromatography.
Alberts, B., and Herrick, G.
Methods in Enzymology, 21, 198-217 (1971)

相关内容

Product Information Sheet

全球贸易项目编号

货号GTIN
D8899-1MG04061833590065
D8899-5MG04061833289525

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