for molecular biology
≥98% (HPLC)
powder
transfection: suitable
H2O: 20 mg/mL (heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.)
PBS: insoluble
30 at 263 nm in H2O at 1 mM
λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
suitable for fluorescence
2-8°C
Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
FPNZBYLXNYPRLR-UHFFFAOYSA-N
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Warning
Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
Respiratory system
11 - Combustible Solids
WGK 3
Not applicable
Not applicable
Eyeshields, Gloves, type N95 (US)
输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。
为方便起见,与您过往购买产品相关的文件已保存在文档库中。
如何查找产品货号
在网站页面上,产品编号会附带包装尺寸/数量一起显示(例如:T1503-25G)。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).
示例
其它示例:
705578-5MG-PW
PL860-CGA/SHF-1EA
MMYOMAG-74K-13
1000309185
输入内容 1.000309185)
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如何查找COA批号
批号可以在产品标签上"批“ (Lot或Batch)字后面找到。
如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。
如果您查询到的批号含有填充代码(例如05427ES-021),请输入去除填充代码-021的批号:05427ES。
如果您查询到的批号含有填充代码(例如 STBB0728K9),请输入去除填充代码K9的批号:STBB0728。
部分情况下,可能未在线提供COA。如果搜索不到COA,可在线索取。
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
This product is soluble in water at 20 mg/mL, but may require heat. DAPI does not dissolve readily in PBS, 0.1 M phosphate, or 0.1 M Tris (pH 7).
Solutions stored in the dark at room temperature have been stable for 2-3 weeks, but solutions exposed to light for the same time period have shown considerable degradation. At 1 mg/mL, solutions are stable in the dark at 2-8 °C for several weeks. For long-term storage, the solutions can be divided into aliquots and stored at -20 °C.
DAPI is used as a nuclear counterstain in our FISH protocol at 2 μg/mL in antifade mounting media. DAPI can also be used as a vital dye to stain mature pollen grains (0.5 μg/mL). DAPI can bind to RNA, but its selectivity for DNA over RNA is high. It appears that DAPI uses an intercalating binding mode with RNA that is AU selective (Biochem., 31, 3103 (1992)). Selectivity of DAPI for DNA over RNA is greater than that shown by ethidium bromide and propidium iodide (Nucleic Acid Research, 6, 3535 (1979))
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Product D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride has a stability of 3 years as supplied. The product in solution is also stable in the dark at 4°C and showed no signs of degradation after three weeks at room temperature in the dark. Material exposed to light showed significant degradation. We have developed an HPLC method for our DAPI products that shows reassay results from July 2012 for lots that were originally released for sale in December 2008, for both D9542 (DAPI dihydrochloride) and D9564 (DAPI dilactate).
Ask a Scientist here.
Regulation of the cell cycle involves processes crucial to the survival of a cell, including the detection and repair of genetic damage as well as the prevention of uncontrolled cell division associated with cancer. The cell cycle is a four-stage process in which the cell 1) increases in size (G1-stage), 2) copies its DNA (synthesis, S-stage), 3) prepares to divide (G2-stage), and 4) divides (mitosis, M-stage). Due to their anionic nature, nucleoside triphosphates (NTPs), the building blocks of both RNA and DNA, do not permeate cell membranes.
可用的荧光原位杂交(FISH)过程、试剂和设备。
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
High titer lentiviral particles for LC3 variants used for live cell analysis of cellular autophagy.
Immunocytochemistry (ICC) Cell Capture Imaging Reagent and protocol for eliminating wash steps, and is highly effective in applications involving rare cells, low cell count samples, and limited access samples.
Step-by-step protocol for generating apical-out human gut organoids for microbiome, ADME/Tox, viral and gastrointestinal related disease research. See the complete organoid culture protocol.
Three-dimensional (3D) printing of biological tissue is rapidly becoming an integral part of tissue engineering.
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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