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Merck
CN

D9542

DAPI

for nucleic acid staining

别名:

4′,6-二脒基-2-苯基吲哚 二盐酸盐, 2-（4-氨基苯基）-6-吲哚甲酰胺 二盐酸盐, DAPI 二盐酸盐

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关于此项目

经验公式（希尔记法）:
C16H15N5 · 2HCl
化学文摘社编号:
28718-90-3
分子量:
350.25
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
24894305
EC Number:
249-186-7
Beilstein/REAXYS Number:
4894417
MDL number:
MFCD00012681

主要文件

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InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

SMILES string

Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

grade

Molecular Biology

assay

≥98% (HPLC)

form

powder

technique(s)

transfection: suitable

solubility

H2O: 20 mg/mL, PBS: insoluble

ε (extinction coefficient)

30 at 263 nm in H2O at 1 mM

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

suitability

suitable for fluorescence

storage temp.

2-8°C

Quality Level

300

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General description

DAPI（4′,6-二脒基-2-苯基吲哚二盐酸盐）是一种可与DNA结合的细胞可通透荧光染料。
可能需要加热或超声处理。溶液可在室温或4 °C下避光稳定保存2至3周。

Application

在琼脂糖凝胶中染色DNA，DAPI比溴化乙锭敏感几倍。
在琼脂糖凝胶中染色DNA，DAPI比溴化乙锭敏感几倍。它可用于DNA的光足迹图谱，通过对双链复合体的特殊可视化检测印迹应用中的退火探针，并使用流式细胞分析术研究DNA中的变化并分析凋亡过程中的DNA含量。DAPI染色也是一种敏感和特异的支原体检测方法。
适用于
  • 琼脂糖凝胶中的DNA染色
  • 分析凋亡过程中DNA的变化
  • 支原体检测
  • DNA的光足迹图谱
  • 细胞的免疫荧光染色

DAPI已用于：
  • 微生物污染的快速监测
  • 染色体条带技术
  • 凋亡细胞的检测
  • 在荧光显微镜中追踪DisA（DNA完整性扫描蛋白）在枯草芽孢杆菌DNA上的移动
  • 对成熟的花粉粒进行染色（0.5 mg/ml）

Biochem/physiol Actions

DNA的细胞渗透性荧光小沟结合探针。与双链DNA的小沟（富含AT的DNA优先）结合，形成稳定的复合物，其发出的荧光比单独的DAPI高约20倍。

Other Notes

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

Disclaimer

避光保存。

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000502555
0000502555
0000496874
0000496874
0000486019

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S Ituarte et al.
Genetica, 122(2), 199-206 (2004-12-22)
Male karyotype and meiosis of Tenagobia fuscata (Corixoidea, Micronectidae) are studied. The species possesses a male diploid chromosome number 2n = 28 + XY, holokinetic chromosomes, absence of m chromosomes and an achiasmatic male meiosis. Autosomes divide pre-reductionally while the
Christy A Itoga et al.
The Journal of comparative neurology, 527(15), 2474-2487 (2019-03-13)
Corticotropin-releasing hormone (CRH) is an essential, evolutionarily-conserved stress neuropeptide. In addition to hypothalamus, CRH is expressed in brain regions including amygdala and hippocampus where it plays crucial roles in modulating the function of circuits underlying emotion and cognition. CRH+ fibers
J Kapuscinski
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 70(5), 220-233 (1995-09-01)
DAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids. The physicochemical properties of the dye and
Yan Li et al.
Toxicology letters, 154(3), 225-233 (2004-10-27)
Excessive exposure to synthetic and endogenous estrogens has been associated with the development of cancer in several tissues including the breast. 4-Hydroxyequilenin (4-OHEN), a major catechol metabolite of equine estrogens present in Premarin, an estrogen replacement formulation, has been shown
Michal Bejerano-Sagie et al.
Cell, 125(4), 679-690 (2006-05-23)
In response to DNA damage, cells activate checkpoint signaling cascades to control cell-cycle progression and elicit DNA repair in order to maintain genomic integrity. The sensing and repair of lesions is critical for Bacillus subtilis cells entering the developmental process
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