Quality Segment
form
liquid
usage
1000 uses (0.5 mg size), 200 uses (0.1 mg size)
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s pBR322 Hae III Digest contains 22 fragments from 8 - 587 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Application
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
Preparation Note
Plasmid pBR322 DNA was digested with Hae III to completion, then purified by phenol extraction.
Other Notes
Contains 22 fragments, 8–587 bp
For optimal resolution, the recommended agarose gel concentration is 2.5.
Sigma′s pBR322 Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable