一般描述
The Plant Protoplast Digest/Wash Solution is formulated to facilitate the rapid isolation of viable protoplasts from plant tissue. Plant cells are surrounded by a rigid, semi-permeable cell wall composed primarily of three classes of polysaccharides: cellulose, hemicellulose, and pectin. The Plant Protoplast Digest/Wash Solution can be used for the digestion of the cell wall after the addition of enzymes that hydrolyze these polysaccharides, such as cellulase, pectinase, or pectolyase. The Plant Protoplast Digest/Wash Solution can be subsequently used to wash away any remaining hydrolytic enzymes after digestion is complete.
Plant protoplasts are typically used for any of a number of downstream applications. These applications include, but are not limited to, transient gene expression, viral transfection assays, somatic hybridization, electrophysiological studies, and morphological studies.
Plant protoplasts are typically used for any of a number of downstream applications. These applications include, but are not limited to, transient gene expression, viral transfection assays, somatic hybridization, electrophysiological studies, and morphological studies.
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Anna Kärkönen et al.
Methods in molecular biology (Clifton, N.J.), 715, 1-20 (2011-01-12)
Plant tissue cultures are an efficient system to study cell wall biosynthesis in living cells in vivo. Tissue cultures also provide cells and culture medium where enzymes and cell wall polymers can easily be separated for further studies. Tissue cultures
Vera Bandmann et al.
Molecular plant, 4(2), 241-251 (2010-12-08)
To analyze the kinetics and size of single exo- and endocytotic events in BY-2 protoplasts, we employed cell-attached membrane capacitance measurements. These measurements revealed different modes of fusion and fission of single vesicles. In about half of the observed exocytotic
Shiu-Cheung Lung et al.
Plant cell reports, 30(4), 473-484 (2010-11-26)
Although transient gene expression using reporters such as green fluorescent protein is a versatile tool for examining gene functions and intracellular protein trafficking, the establishment of a highly efficient gene manipulation method remains a challenge in many plant species. A
The cell wall.
Carpita, N., and McCann, M. et al.
Biochemistry, 52-108 null
Akira Inoue et al.
Marine biotechnology (New York, N.Y.), 13(2), 256-263 (2010-04-16)
Functional recombinant abalone alginate lyase (rHdAly) and β-1,4-endoglucanase (rHdEG66) were expressed as secreted proteins with baculoviral expression systems. The specific activity of each recombinant enzyme, 2,490 and 18.2 U/mg for rHdAly and rHdEG66, respectively, was comparable to its native form
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持