正在寻找类似产品? 访问 产品对比指南
一般描述
质粒pBR322是构建用于大肠埃希菌的首批多用途克隆载体之一。该质粒衍生自ColE1型质粒pMB1,并具有与ColE1及其亲属相同的复制机制和对照。 质粒pBR322赋予对氨苄青霉素和四环素的抗性。质粒序列已发表。
质粒在氨苄西林耐药基因(Pst I、Pvu I和Sca I)、四环素耐药基因(BamH I、BspM I、EcoR V、Nhe I、Nru I、Sal I、Sph I和Xma III)和其他基因(Aat II、Ava I、Bal I、Bsm I、BspM II、Cla I、EcoR I、Hind III、Nde I、Pvu II、Ssp I、Sty I和Tth111 I)内具有独特的酶切位点。
质粒在氨苄西林耐药基因(Pst I、Pvu I和Sca I)、四环素耐药基因(BamH I、BspM I、EcoR V、Nhe I、Nru I、Sal I、Sph I和Xma III)和其他基因(Aat II、Ava I、Bal I、Bsm I、BspM II、Cla I、EcoR I、Hind III、Nde I、Pvu II、Ssp I、Sty I和Tth111 I)内具有独特的酶切位点。
特异性
唯一位点:在氨苄西林抗性基因内:Pst I,Pvu I,Sca I;在四环素抗性基因内:BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, Xma III;其他唯一位点:Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, Tth111 I.
应用
从大肠埃希菌中提取的pBR322质粒DNA RRI已被用作低分子量DNA,用于测试翡翠贻贝部分纯化组分的酶活性。它还被用于通过扫描电子显微镜研究考察其对碳酸钙颗粒形态的作用。
质粒pBR322是构建用于大肠埃希菌的首批多用途克隆载体之一。 该质粒和衍生物已用于许多目的,包括克隆、选择和表达重组分子,构建穿梭载体和用于核苷酸测序的载体、研究与基因表达有关的元素、作为质粒DNA标准品和模型系统,用于原核质粒复制的研究。
生化/生理作用
该质粒衍生自ColE1型质粒pMB1,并具有与ColE1及其亲属相同的复制机制和对照。
组分
DNA以粉末形式提供,由1 mM Tris-HCl (pH 7.5) + 1 mM NaCl和1 mM EDTA溶液冻干。
其他说明
登记号:J01749
登记号:J01749
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
Changes in tumorigenesis- and angiogenesis-related gene transcript abundance profiles in ovarian cancer detected by tailored high density cDNA arrays.
Cancer Biochemistry Biophysics, 6, 750-750 (2000)
PLoS genetics, 4(11), e1000259-e1000259 (2008-11-15)
Dishevelled (Dvl) proteins are important signaling components of both the canonical beta-catenin/Wnt pathway, which controls cell proliferation and patterning, and the planar cell polarity (PCP) pathway, which coordinates cell polarity within a sheet of cells and also directs convergent extension
Journal of chromatography. A, 972(1), 77-86 (2002-10-25)
A HPLC technique has been developed, based on hydrophobic-interaction Chromatography with a non-porous packing (TSKgel Butyl-NPR, Tosoh Biosep LLC), that allows separation of the open circular (nicked) and supercoiled forms of five DNA plasmids, ranging in size from 4 to
Journal of colloid and interface science, 352(1), 43-49 (2010-09-11)
Calcium carbonate microspheres with different surface structures were successfully prepared by the reaction of sodium carbonate with calcium chloride in the presence of deoxyribonucleic acid (DNA) at room temperature. The as-prepared products were characterized by scanning electron microscopy (SEM), transmission
Indian journal of experimental biology, 54(12), 816-821 (2016-12-01)
Viral diseases are not only responsible for health related issues but also exert pressure on the State economy. Tropical and subtropical countries have more prevalence of virus associated pathological conditions such as chickenpox, adenovirus related infections, dengue, chickengunya, infectious mononucleosis
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门