grade
Molecular Biology
Quality Segment
form
lyophilized powder
technique(s)
Southern blotting: suitable
foreign activity
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.
2-8°C
General description
Denhardt溶液是一种阻断剂,通常用于减少检测试剂与Northern和Southern印迹膜的非特异性结合。该粉末可用于制备50倍浓缩液,可稀释至任何浓度,用于各种预杂交和杂交缓冲液。
Application
适合用于Southern和Northern印迹膜的封闭缓冲液。
Preparation Note
制备50倍浓缩液,将全部内容物溶解在5 mL分子生物级水中。在-20°C下可储存长达两年。
每管Denhardt粉末含有50mg牛血清白蛋白(BSA)、50mg Ficoll和50mg聚乙烯吡咯烷酮(PVP)。
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存储类别
11 - Combustible Solids
怎么回事?
WGK 3
闪点 (F)
Not applicable
闪点 (°C)
Not applicable
PPE(个人防护设备)
Eyeshields, Gloves, type N95 (US)