等级
Molecular Biology
质量水平
表单
lyophilized powder
技术
Southern blotting: suitable
异质活性
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
储存温度
2-8°C
一般描述
Denhardt溶液是一种阻断剂，通常用于减少检测试剂与Northern和Southern印迹膜的非特异性结合。该粉末可用于制备50倍浓缩液，可稀释至任何浓度，用于各种预杂交和杂交缓冲液。
应用
Denhardt溶液已被用于：
- 作为杂交缓冲液的组分，用于脑切片的杂交
- 用于原位杂交过氧化物酶-（抗过氧化物酶法-全标本包埋）（ISHPAP-WM）过程
- 用于杂交混合物的制备
适合用于Southern和Northern印迹膜的封闭缓冲液。
生化/生理作用
Denhardt溶液和明胶-铬明矾可用于处理染色体，从而在原位杂交后用胰蛋白酶处理以实现染色体的g显带。
制备说明
制备50倍浓缩液，将全部内容物溶解在5 mL分子生物级水中。在-20°C下可储存长达两年。
每管Denhardt粉末含有50mg牛血清白蛋白（BSA）、50mg Ficoll和50mg聚乙烯吡咯烷酮（PVP）。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
含少量动物源组分生物产品
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Kazuya Morikawa et al.
PLoS pathogens, 8(11), e1003003-e1003003 (2012-11-08)
It has long been a question whether Staphylococcus aureus, a major human pathogen, is able to develop natural competence for transformation by DNA. We previously showed that a novel staphylococcal secondary sigma factor, SigH, was a likely key component for
Andreas Toegl et al.
Journal of biomolecular techniques : JBT, 14(3), 197-204 (2003-09-19)
Protein and DNA microarrays have become a standard tool in proteomics/genomics research. In order to guarantee fast and reproducible hybridization results, the diffusion limit must be overcome. Surface acoustic wave (SAW) micro-agitation chips efficiently agitate the smallest sample volumes (down
M A Hull et al.
Gut, 43(4), 525-536 (1998-11-21)
Basic fibroblast growth factor (bFGF) promotes angiogenesis and healing of gastric ulcers in rats, and bFGF expression is up regulated in such ulcers. However, little is known about expression of bFGF in human gastric mucosa. To investigate bFGF expression in
M Simpson-Holley et al.
Journal of virology, 85(4), 1581-1593 (2010-12-15)
Vaccinia virus (VV) mutants lacking the double-stranded RNA (dsRNA)-binding E3L protein (ΔE3L mutant VV) show restricted replication in most cell types, as dsRNA produced by VV activates protein kinase R (PKR), leading to eIF2α phosphorylation and impaired translation initiation. Here
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