General description
通过下文命名的 Sigma 试剂盒标准方案中提供的程序，将绝大多数 DNA 从总 RNA 纯化中去除。然而，对于非常灵敏的应用，如定量 RT-PCR，即使微量的 DNA 也会导致 cDNA 来源扩增子的假阳性检测。
柱上 DNase I 消化套装为从总 RNA 制备物中去除基因组 DNA 提供了一种简单方便的方法。本套装拟与 GenElute ™ Mammalian Total RNA Miniprep Kit、GenElute Bacterial Total RNA Purification Kit、GenElute Yeast Total RNA Purification Kit 以及 Spectrum™ Plant Total RNA Kit配套使用。不过酶切方案与许多其他市售的 RNA 纯化试剂盒兼容。
术语“柱上”表示消化是在过程中完成的，以确保方便。Sigma的每个总RNA纯化试剂盒都使用结合柱进行纯化。 DNase I消化在15分钟内完成，同时总RNA与柱底物结合。 随后立即进行几次洗涤步骤和总RNA的洗脱。 要查看完整的方案，请参阅上面列出的任何工具包的技术公告。
柱上 DNase I 消化套装为从总 RNA 制备物中去除基因组 DNA 提供了一种简单方便的方法。本套装拟与 GenElute ™ Mammalian Total RNA Miniprep Kit、GenElute Bacterial Total RNA Purification Kit、GenElute Yeast Total RNA Purification Kit 以及 Spectrum™ Plant Total RNA Kit配套使用。不过酶切方案与许多其他市售的 RNA 纯化试剂盒兼容。
术语“柱上”表示消化是在过程中完成的，以确保方便。Sigma的每个总RNA纯化试剂盒都使用结合柱进行纯化。 DNase I消化在15分钟内完成，同时总RNA与柱底物结合。 随后立即进行几次洗涤步骤和总RNA的洗脱。 要查看完整的方案，请参阅上面列出的任何工具包的技术公告。
柱上 DNase I 消化套装为从总 RNA 制备物中去除基因组 DNA 提供了一种简单方便的方法。本套装拟与 GenElute ™ Mammalian Total RNA Miniprep 试剂盒和 Spectrum Plant Total RNA 试剂盒配套使用。它也可以与许多其他市售的 RNA 纯化试剂盒一起使用。
Application
柱上 DNase I 酶切套装适用于去除总 RNA 制备物中的 gDNA。它可能不适合 RT-PCR 应用，因为可能残留少量残留 DNA。用柱上 DNaseⅠ 消化套装纯化葡萄品种的 RNA。
Biochem/physiol Actions
为方便起见，在过程中完成柱上消化。DNase I 消化在 15 min 内完成，而总 RNA 与柱底物结合。随后立即进行几次洗涤步骤和总 RNA 洗脱。
Features and Benefits
DNase Ⅰ柱上消化试剂盒提供了一种从总 RNA 制剂中去除基因组 DNA的简便方法。此试剂盒设计与 GenElute™ 哺乳动物总 RNA 小提试剂盒、GenElute 细菌总 RNA 纯化试剂盒、GenElute 酵母总 RNA 纯化试剂盒和 Spectrum™ 植物总 RNA 试剂盒配合使用。但其消化方案与许多其他市售 RNA 纯化试剂盒兼容。
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
Spectrum is a trademark of Sigma-Aldrich Co. LLC
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
10 - Combustible liquids
wgk
WGK 3
法规信息
动植物源性产品
低风险生物材料
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