Merck
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DNASE70

Sigma-Aldrich

柱上 DNase I 消化套装

sufficient for 70 preparations

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别名:
脱氧核糖核酸酶 Ⅰ 套装
NACRES:
NA.52

等级

for molecular biology

质量水平

形式

liquid

用途

sufficient for 70 preparations

technique(s)

RNA purification: suitable

储存温度

−20°C

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此商品
DNASE10AMPD111284932001
DNase I Amplification Grade

Sigma-Aldrich

AMPD1

脱氧核糖核酸酶I

DNase I from bovine pancreas

Roche

11284932001

DNase I

form

liquid

form

liquid

form

liquid

form

lyophilized

technique(s)

RNA purification: suitable

technique(s)

RNA purification: suitable

technique(s)

RT-PCR: suitable

technique(s)

cell culture | mammalian: suitable

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

usage

sufficient for 70 preparations

usage

sufficient for 10 preparations

usage

-

usage

-

一般描述

柱上 DNase I 消化套装为从总 RNA 制备物中去除基因组 DNA 提供了一种简单方便的方法。本套装拟与 GenElute Mammalian Total RNA Miniprep 试剂盒和 Spectrum Plant Total RNA 试剂盒配套使用。它也可以与许多其他市售的 RNA 纯化试剂盒一起使用。
通过下文命名的 Sigma 试剂盒标准方案中提供的程序,将绝大多数 DNA 从总 RNA 纯化中去除。然而,对于非常灵敏的应用,如定量 RT-PCR,即使微量的 DNA 也会导致 cDNA 来源扩增子的假阳性检测。

柱上 DNase I 消化套装为从总 RNA 制备物中去除基因组 DNA 提供了一种简单方便的方法。本套装拟与 GenElute Mammalian Total RNA Miniprep Kit、GenElute Bacterial Total RNA Purification Kit、GenElute Yeast Total RNA Purification Kit 以及 Spectrum Plant Total RNA Kit配套使用。不过酶切方案与许多其他市售的 RNA 纯化试剂盒兼容。

术语“柱上”表示消化是在过程中完成的,以确保方便。Sigma的每个总RNA纯化试剂盒都使用结合柱进行纯化。 DNase I消化在15分钟内完成,同时总RNA与柱底物结合。 随后立即进行几次洗涤步骤和总RNA的洗脱。 要查看完整的方案,请参阅上面列出的任何工具包的技术公告。

应用

柱上 DNase I 酶切套装适用于去除总 RNA 制备物中的 gDNA。它可能不适合 RT-PCR 应用,因为可能残留少量残留 DNA。用柱上 DNaseⅠ 消化套装纯化葡萄品种的 RNA。

特点和优势

柱上 DNase I 消化套装为从总 RNA 制备物中去除基因组 DNA 提供了一种简单方便的方法。本套装拟与 GenElute Mammalian Total RNA Miniprep Kit、GenElute Bacterial Total RNA Purification Kit、GenElute Yeast Total RNA Purification Kit 以及 Spectrum Plant Total RNA Kit配套使用。不过酶切方案与许多其他市售的 RNA 纯化试剂盒兼容。

原理

为方便起见,在过程中完成柱上消化。DNase I 消化在 15 min 内完成,而总 RNA 与柱底物结合。随后立即进行几次洗涤步骤和总 RNA 洗脱。

法律信息

GenElute is a trademark of Sigma-Aldrich Co. LLC
Spectrum is a trademark of Sigma-Aldrich Co. LLC

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

10 - Combustible liquids

WGK

WGK 3


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Ghazanfar A Chishti et al.
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Transition to grain increases inflammation and causes parakeratosis, which can decrease growth performance in fattening animals. It is unknown if ruminants adapt to these inflammatory responses over time. In a three-phase, 49-day experiment, all wethers (n = 13, BW =
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