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一般描述
脱氧核苷酸三磷酸(dNTP)是带负电荷的有机分子,其中包含与脱氧核糖结合的核碱基。该dNTPs组包括在单独的试管中的四种脱氧核糖核苷酸(即脱氧核糖腺苷三磷酸(dATP),脱氧核糖胞苷三磷酸(dCTP),脱氧核糖鸟苷三磷酸(dGTP)和脱氧核糖胸苷三磷酸(dTTP))的100mM水溶液各1.0 mL,以方便和容易地进行PCR反应设置。
应用
100 mM脱氧核苷酸套件用于:
- 聚合酶链式反应(PCR)中的DNA的扩增
- 定量PCR(qPCR)
- 多重PCR
生化/生理作用
脱氧核苷酸三磷酸(dNTP)是核酸分子的合成砌块,呈酸性。作为DNA聚合酶的底物。dNTP是PCR预混液的基本成分,因为它是DNA扩增反应所必需的。理想的dNTP含量可确保适当的染色体复制,进而保持基因组稳定性。
特点和优势
- 提供4管分开的dNTP
- 允许优化单个dNTP浓度
包装
100mM dATP、dCTP、dGT和dTTP,各1.0 mL
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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