E0639
内切糖苷酶F2 from Elizabethkingia miricola
recombinant, expressed in E. coli, 20 U/mg
别名:
内-β-N-乙酰氨基葡糖苷酶F2，Endo F2, 内切糖苷酶F2 来源于脑膜炎脓毒性黄杆菌, 内切糖苷酶F2 来源于脑膜脓毒性伊丽莎白菌, 内切糖苷酶F2 来源于脑膜脓毒性金黄杆菌, 米尔伊丽莎白菌
产品名称
内切糖苷酶F2 from Elizabethkingia miricola, recombinant, expressed in E. coli, 20 U/mg
recombinant
expressed in E. coli
conjugate
(N-linked)
form
solution
specific activity
20 U/mg
mol wt
32 kDa
shipped in
wet ice
storage temp.
2-8°C
Quality Level
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Other Notes
一个单元将在37 °C、pH 4.5下每分钟从1 μmole变性猪纤维蛋白原释放N-连接寡糖。
Packaging
提供 5× 反应缓冲液，250 mM 醋酸钠，pH 4.5
Physical form
无菌灌装溶液，含10 mM乙酸钠、25 mM氯化钠，pH 4.5
存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
D Wade Abbott et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 67(Pt 4), 429-433 (2011-04-21)
EndoD is an architecturally complex endo-β-1,4-N-acetylglucosamidase from Streptococcus pneumoniae that cleaves the chitobiose core of N-linked glycans and contributes to pneumococcal virulence. Although the glycoside hydrolase family 85 catalytic module has been structurally and functionally characterized, nothing is known about
Midori Umekawa et al.
Biochimica et biophysica acta, 1800(11), 1203-1209 (2010-07-22)
An efficient method for synthesizing homogenous glycoproteins is essential for elucidating the structural and functional roles of glycans of glycoproteins. We have focused on the transglycosylation activity of endo-ß-N-acetylglucosaminidase from Mucor hiemalis (Endo-M) as a tool for glycoconjugate syntheses, since
Roger S Zou et al.
Aging, 3(10), 968-984 (2011-10-13)
A distinct conformational transition from the α-helix-rich cellular prion protein (PrPC) into its β-sheet-rich pathological isoform (PrPSc) is the hallmark of prion diseases, a group of fatal transmissible encephalopathies that includes spontaneous and acquired forms. Recently, a PrPSc-like intermediate form
Eric M Rubenstein et al.
The Journal of cell biology, 197(6), 761-773 (2012-06-13)
Little is known about quality control of proteins that aberrantly or persistently engage the endoplasmic reticulum (ER)-localized translocon en route to membrane localization or the secretory pathway. Hrd1 and Doa10, the primary ubiquitin ligases that function in ER-associated degradation (ERAD)
Yusuke Tomabechi et al.
Carbohydrate research, 345(17), 2458-2463 (2010-10-12)
To determine the structural specificity of the glycosyl acceptor of the transglycosylation reaction using endo-β-N-acetylglucosaminidase (ENGase) (EC 3.2.1.96) from Mucor hiemalis (Endo-M), several acceptor derivatives were designed and synthesized. The narrow regions of the 1,3-diol structure from the 4- to
商品
Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
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