产品名称
内皮细胞生长补充剂 来源于牛脑垂体, ECGS, suitable for cell culture, BioReagent
biological source
bovine pituitary glands
product line
BioReagent
form
powder
potency
2-300 μg/mL ED50/EC50
quality
endotoxin tested
packaging
pkg of 15 mg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤20 EU/vial (LAL test)
color
brown to tan
solubility
water: soluble to hazy, red to red-brown (1 vial reconstituted with 1 ml 0.85% NaCl)
storage temp.
2-8°C
Quality Level
General description
内皮细胞生长补充剂（ECGS）是一种包含哺乳动物血管内皮细胞生长促进因子的牛垂体腺的提取物。内皮细胞生长补充剂使用一种已发表方法的改良方案制备而成。它通过从含有NaCl和硫酸链霉素的无菌溶液冻干而成。
Application
牛垂体内皮细胞生长补充剂已用作：
- 罗斯威尔公园纪念研究所（RPMI）1640培养中中用于内皮细胞培养的组成成分
- 内皮细胞培养培养基199的组成成分
- 杜氏′改良Eagle培养基（DMEM）：Ham′s F-12的补充剂
- Iscove改良DMEM培养基（IMDM）用于人脐静脉内皮细胞（HUVEC）培养的补充剂′′
Biochem/physiol Actions
据报道，内皮细胞生长补充剂（ECGS）可作为培养基补充剂在单克隆抗体生产的杂交瘤细胞融合和生长中发挥积极作用。
signalword
Warning
hcodes
Hazard Classifications
Repr. 2
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
监管及禁止进口产品
此项目有
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Autoantibodies against heat shock protein 60 mediate endothelial cytotoxicity.
Schett G, et al.
The Journal of Clinical Investigation, 96(6), 2569-2577 (1995)
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Xu Q, et al.
Circulation Research, 75(6), 1078-1085 (1994)
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