E0887
酯酶 来源于兔肝脏
lyophilized powder, ≥30 units/mg protein
别名:
羧酸酯水解酶, 羧酸酯酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-773-7
MDL number:
Specific activity:
≥30 units/mg protein
form
lyophilized powder
Quality Level
specific activity
≥30 units/mg protein
mol wt
60 kDa
composition
Protein, ≥85% biuret
storage temp.
−20°C
General description
酯酶属于C族羧酸酯酶。它是一种糖蛋白，主要存在于肝细胞的内质网中。
Application
Sigma的酶产品已被用于研究二价金属离子对酯酶活性的影响。
兔肝酯酶已被用于：
- 通过乙酸乙烯酯、三酰甘油、对-硝基苯基酯和丝氨酸酶抑制剂检测进行牛胆盐激活脂肪酶的比较生物物理研究
- 肽类化合物的催化和动力学分析研究
- 前药化合物（BMS-292655和BMS300043）的体外稳定性测定
- 橄榄苦苷元（oleuropein aglycone，Ole-AG）的水解
来自兔肝脏的酯酶已被用于研究氨基甲酸酯类杀虫剂的毒性作用。兔肝酯酶还被用于研究辛伐他汀对脂蛋白相关磷脂酶A(2) 的表达和活性的影响。
Biochem/physiol Actions
酯酶具有广泛的底物特异性。兔肝酯酶已被用于研究氨基甲酸酯类杀虫剂的毒性作用。兔肝酯酶已被用于辛伐他汀的水解。
酯酶在水存在的情况下催化羧酸酯水解为羧酸盐和醇。兔肝羧酸酯酶催化桉油酚衍生物的对映选择性水解。
Physical form
含 Tris 缓冲盐的冻干粉
Other Notes
一个酶活性单位是指在pH 8.0、25℃条件下每分钟将1.0 μM丁酸乙酯水解成丁酸和乙醇所需的酶量。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
低风险生物材料
此项目有
实验方案
Objective: To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.
在测定酯酶活性时，使用丁酸乙酯通过pH滴定速率法进行测定。酯酶可以将酯水解成酸和醇。
Shijin Zhang et al.
Langmuir : the ACS journal of surfaces and colloids, 36(22), 6261-6267 (2020-05-19)
To explore the potential of step-by-step assembly in the fabrication of biological materials, we designed and synthesized two peptide-based molecules for enzyme-instructed hierarchical assembly. Upon the treatment of alkaline phosphatase, one molecule undergoes enzyme-instructed self-assembly forming uniformed nanofibers. The other
Henri Chahinian et al.
Biochimica et biophysica acta, 1801(11), 1195-1204 (2010-07-27)
To differentiate esterases from lipases at the structure-function level, we have compared the kinetic properties and structural features of sequence-related esterase 1 from rabbit liver (rLE) and bile-salt-activated lipase from bovine pancreas (bBAL). In contrast to rLE, bBAL hydrolyses water-insoluble
Anna Sobeková et al.
Acta biologica Hungarica, 60(1), 45-54 (2009-04-22)
The activities of the antioxidant and detoxifying enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx), glutathione reductase (GR), glutathione-S-transferase (GST), and the content of thiobarbituric acid reactive substances (TBARS) were determined in the liver and kidney of rabbits after
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| E0887-500UN | 04061832755540 |
| E0887-1KU | 04061833601297 |