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Molecular Biology
form
buffered aqueous glycerol solution
mol wt
28 kDa
UniProt accession no.
storage temp.
−20°C
Gene Information
Escherichia coli K12 ... xthA(946254)
General description
一种可催化从dsDNA的3′-末端进行单核苷酸去除的3′→5′核酸外切酶。该酶还具有嘌呤和嘧啶内切核酸酶、3′-DNA磷酸酶和RNase H活性。活性高度依赖于温度、盐浓度以及酶与DNA的比例，因此反应条件必须针对特定应用进行优化。
Application
适用于：
- 生产链特异性探针
- 用于Sanger双脱氧测序的单链模板的制备
- 定点诱变
Other Notes
在37 °C下，一个单位可在30分钟内可从经超声处理的小牛胸腺DNA中释放1 nmole的酸可溶性核苷酸。
核酸外切酶III是以溶于5 mM 磷酸钾（pH 6.5）、200 mM KCl、0.05 mM EDTA、5 mM 2- 巯基乙醇、200 μg/ml BSA和50%甘油的溶液形式提供的。
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
L H Guo et al.
Nucleic acids research, 10(6), 2065-2084 (1982-03-25)
We describe improve enzymatic methods for sequencing method for sequencing DNA. They are based on partial digestion of duplex DNA with exonuclease III to produce DNA molecules with 3' ends shortened to varying lengths, followed by repair synthesis to extend
Ming Luo et al.
Chemical communications (Cambridge, England), 48(59), 7416-7418 (2012-06-21)
An amplified multiplexed DNA detection biosensor has been developed, which combines the unique cleavage function of exonuclease III (Exo III) with the separating ability of magnetic microparticles (MMPs). By using different fluorophores, the multiplexed detection of DNA is demonstrated.
Sai Bi et al.
Chemical communications (Cambridge, England), 48(7), 1018-1020 (2011-12-07)
The network consisting of three kinds of unlabeled stem-loop DNA molecular beacons (MBs) is activated by target DNA in the presence of exonuclease-III (Exo-III), achieving the concept of exonuclease-assisted cascaded recycling amplification (Exo-CRA) for DNA detection with a wide dynamic
Ying Chen et al.
Chemical communications (Cambridge, England), 47(48), 12798-12800 (2011-11-15)
The presence of exonuclease III leads to direct recycling and reuse of the target DNA, which in turn results in substantial signal amplification for highly sensitive, label-free impedimetric detection of specific DNA sequences.
Peng Hu et al.
Biosensors & bioelectronics, 34(1), 83-87 (2012-03-03)
We report here a graphene oxide (GO)-based fluorescent aptasensor for adenosine detection by employing exonuclease III (Exo III) as a signal amplifying element. In the absence of adenosine, the adenosine aptamers hybridized with the complementary DNA (cDNA), and the Exo
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