E1257
表皮生长因子 来源于鼠颔下腺
lyophilized powder, BioReagent, suitable for cell culture
别名:
EGF, 表皮细胞生长因子
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关于此项目
biological source
mouse submaxillary glands
Quality Segment
product line
BioReagent
assay
≥98% (SDS-PAGE)
form
lyophilized powder
potency
0.02-2.0 ng/mL EC50
packaging
pkg of 0.1 mg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
color
white
UniProt accession no.
storage temp.
2-8°C
Gene Information
mouse ... Egf(13645)
General description
Epidermal growth factor (EGF) is the member of EGF-like family of growth factors, that binds to EGF receptor. It is majorly expressed in kidney, parotid gland and salivary gland.
Application
Epidermal Growth Factor from murine submaxillary gland has been used
- in studying its effects on the development of caustic esophageal stricture in rats
- for preparation of MCF-10A cell line
- for the culture of primary hair follicle stem cells from arbas cashmere goat (gHFSCs) and adhesion of hair follicles (HF)
Biochem/physiol Actions
Epidermal growth factor (EGF) blocks gastric acid and pepsin secretion and increases gastrointestinal cell proliferation. EGF stimulates proliferation and migration in bone marrow stromal cells (BMSC), that gives rise to osteoblasts, chondrocytes and adipocytes. It functions as a strong mitogenic factor for various cultured cells. Mutations in this gene is linked to hypomagnesemia. EGF plays a major role in the maintenance of hair and skin development.
Physical form
Lyophilized from 0.2 μm filtered solution in ammonium acetate buffer.
Preparation Note
Purified by gel filtration and ion-exchange chromatography.
Analysis Note
The biological activity is measured in a proliferation assay using BALB/MK cells.
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存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)