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E1270

ECM 凝胶 来源于 Engelbreth-Holm-Swarm 小鼠肉瘤

liquid, BioReagent, suitable for cell culture

别名:

EHS基质

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关于此项目

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00283131
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product line

BioReagent

Quality Level

300

form

liquid

concentration

8-12 mg/mL

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

P02468, P02469, P10493, P19137, P43407, Q61001, Q61092, Q61789

shipped in

dry ice

storage temp.

−20°C

Gene Information

mouse ... Lama1(16772), Lama3(16774), Lama5(16776), Lamb1-1(16777), Lamc1(226519), Lamc2(16782), Nid1(18073), Sdc2(15529)

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相关类别

Application

ECM凝胶可用于上皮细胞、内皮细胞、肌肉细胞、神经细胞和肿瘤细胞。

Biochem/physiol Actions

当达到20-40°C时，ECM基质胶将发生热活化聚合，形成重组基底膜。 当PC12细胞在该ECM胶薄层上生长时，将在2天内形成神经突。

用于生产本品的每个小鼠种群都定期筛查病原体。经检测，LDEV呈阴性。

Preparation Note

该产品是从小鼠Engelbreth-Holm-Swarm肉瘤中提取的；并经过氯仿透析。使用前，将凝胶置于2-8℃下解冻过夜，并使用预冷至2-8℃的板和移液器将凝胶分配至多孔板。也可使用2-8℃的Dulbecco改良Eagle培养基将凝胶稀释最多两倍，应先稀释、再分配至多孔板。 该产品在20°C下放置5分钟即可成胶。对于长时间操作，应在低于10°C的温度下进行。细胞可接种于0.5 mm厚的凝胶层上或在1mm厚的凝胶层内培养。 如果在凝胶层内培养细胞，请在接种前将细胞加至凝胶中，建议密度为3-4 x 104 个细胞/mL。 可使用溶剂为PBS，不含钙、镁或EDTA且浓度为0.6-2.4单位/mL的蛋白酶，将细胞从凝胶中分离出来。

Other Notes

ECM制剂的蛋白质浓度为8-12 mg/mL（DMEM溶液），主要成分为层粘连蛋白，还含有IV型胶原蛋白、蛋白多糖硫酸乙酰肝素、巢蛋白等其他次要成分。向ECM凝胶中添加IV型胶原蛋白可增加其聚合度。

本品含有50 mg/L硫酸庆大霉素。

Disclaimer

如需长期储存，请将本品置于-20°C储存。ECM凝胶可在2-8℃下储存最长72小时。

存储类别

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

动物来源培养基

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000522423
0000522422
0000522421
0000522423
0000522422

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全球贸易项目编号

货号GTIN
E1270-1ML04061833601600
E1270-10ML04061833601594
E1270-5ML04061838447104
E1270-5X10ML04061833601617

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