E1388
β-内皮细胞生长因子 人
≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
别名:
ECGS, β-ECGF
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关于此项目
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥97% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
predicted mol wt ~17 kDa
Impurities:
≤1.0 EU/μg protein Endotoxin level (LAL test)
产品名称
β-内皮细胞生长因子 人, β-ECGF, recombinant, expressed in E. coli, suitable for cell culture
biological source
human
recombinant
expressed in E. coli
assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
0.03-0.3 ng/mL ED50/EC50
quality
endotoxin tested
mol wt
predicted mol wt ~17 kDa
packaging
pkg of 25 μg
storage condition
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
technique(s)
cell culture | mammalian: suitable
impurities
≤1.0 EU/μg protein Endotoxin level (LAL test)
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
human ... VEGFB(7423)
General description
β-内皮细胞生长因子（b-ECGF）基因编码血管生成因子，并定位于人染色体 11q13.1 上。重组人 b 内皮细胞生长因子（b-ECGF）是酸性成纤维细胞生长因子的 14 个氨基酸 N 端延伸形式。它在大肠杆菌中表达。去除启动蛋氨酸后产生的 154 个氨基酸重组蛋白，预测分子质量约为 17 kDa。
Application
人 A-内皮细胞生长因子可用于：
- 细胞死亡试验
- 内皮细胞培养
- 人脐血管内皮细胞（HUVEC）培养
Biochem/physiol Actions
β-内皮细胞生长因子（β-ECGF）是一种有效的内皮细胞促分裂原，肝素可增强其促有丝分裂活性。β-ECGF 刺激培养的静态 HUVEC 细胞的生长。β-ECGF 与脂质和葡萄糖代谢有关。该生长因子在疾病条件下诱导新血管形成。在糖尿病性视网膜病和多囊卵巢综合征中观察到 β-ECGF 的上调。它还与肿瘤增殖和侵袭有关。β-ECGF 增加脂肪酸转运蛋白的表达，以改善内皮介导的脂质转运。
Physical form
本品是从 0.2 μm 过滤溶液中冻干，溶液含 10 mM MOPS，50 mM 硫酸铵，0.2 mM DTT 和 0.2 mM EDTA，pH 7.2，每 1 μg 细胞因子含 50 μg 牛血清白蛋白。
Analysis Note
通过使用静态 NR6R-3T3 成纤维细胞在培养物中检测增殖活性。
Other Notes
14 个氨基酸，人酸性成纤维细胞生长因子 FGF 的 N 端延伸形式。
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
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