Anti-ELMO (C-terminal) antibody produced in rabbit

ELMO1 (engulfment and cell motility 1) is a member of the evolutionarily conserved family of ELMO proteins.

Anti-ELMO antibody produced in rabbit is suitable for immunoprecipitation at a working antibody amount of 1-2μL using a whole extract of mouse A-20 cells. It is also suitable for immunoblotting at a working dilution of 1:2000-1:3000 using a whole extract of human Jurkat cells and at 1:1000-1:2000 using a whole extract of rat brain.

ELMO proteins regulate actin cytoskeleton reorganization during engulfment and cell migration. ELMO1 and Dock180 function together as a guanine exchange factor (GEF) for the small GTPase Rac, leading to its activation and cytoskeleton rearrangement. The C-terminal PH domain of ELMO1 binds to Dock180 and the N-terminal binds to the active form of the small GTPase RhoG, targeting the ELMO/Dock180 complex to the membrane. It also interacts with BAI1, an engulfment receptor for apoptotic cells upstream of the ELMO1/Dock180/Rac module. Increased expression of ELMO1 and Dock180 are associated with the invasive phenotype of glioma cells.

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

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