Monoclonal Anti-Endonuclease VIII antibody produced in mouse

Monoclonal Anti- Endonuclease VIII (mouse IgM isotype) is derived from the E8-122 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice . Endonuclease VIII (Endo VIII) from E. coli is a product of the nei gene. It is a DNA N-glycosylase and abasic site lyase (AP1 lyase. It comprises C-terminal Cys4-type β/β-antiparallel zinc finger with an arginine residue (Arg-252).

recombinant full length endonuclease VIII from Escherichia coli.

Monoclonal Anti-Endonuclease VIII antibody produced in mouse may be used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.

Endonuclease VIII (Endo VIII) from E. coli is a DNA repair protein that recognizes and removes modified pyrimidines, such as thymine glycol (Tg) and 5,6,dihydrothymine (DHT), from DNA.. Its modified base substrate specificity overlaps the endonuclease III (endo III, nth) substrate specificity. This cross-reactivity is manifested in E. coli mutants. E. coli nth and nei mutants are either not sensitive or are slightly more sensitive to ionizing radiation and hydrogen peroxides than the wild type. The nei-nth double mutant is hypersensitive to oxidative stress. The mechanism used by Endo VIII to cleave the DNA backbone (lyase activity) is a β-δ elimination, resembling the action of formamidopyrimidine-DNA glycosylase (fpg) protein.

Monoclonal Anti-Endonuclease VIII recognizes endonuclease VIII from E. coli (approx. 30 kDa).

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.

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