Product Name
内皮细胞生长补充剂 来源于牛神经组织, ECGS, suitable for cell culture
生物来源
bovine neuroblastoma
质量水平
表单
powder
效能
2-200 μg/mL ED50/EC50
质量
endotoxin tested
包装
pkg of 15 and 5X15 mg
储存条件
avoid repeated freeze/thaw cycles
技术
cell culture | mammalian: suitable
杂质
≤20 EU/vial (LAL test)
颜色
tan to brown
溶解性
water: soluble (1 vial reconstituted with 1 ml 0.85% NaCl)
储存温度
2-8°C
相关类别
一般描述
内皮细胞生长补充剂(ECGS)包含酸性成纤维细胞生长因子(aFGF)和碱性FGF(bFGF)。
应用
来自牛神经组织的内皮细胞生长补充剂已作为杜氏′改良Eagles′培养基补充剂和F-12K培养基补充剂,用于人脐静脉内皮细胞培养。
生化/生理作用
内皮细胞生长补充剂(ECGS)可改善人隐静脉内皮细胞(HSVEC)和人脐静脉内皮细胞(HUVEC)的增殖。它可与白介素1(IL-1)协调并介导血管平滑肌细胞(VSMC)中的基质重塑。它对于内皮细胞增殖和脂肪干细胞(ASC)的分化至关重要。包含哺乳动物血管内皮细胞生长促进因子的牛神经组织提取物。据报道,ECGS可作为培养基补充剂在单克隆抗体生产的杂交瘤细胞融合和生长中发挥积极作用。
制备说明
ECGS是一种包含哺乳动物血管内皮细胞生长促进因子的牛神经组织提取物。内皮细胞生长补充剂是通过对Maciag等人的方法进行修改而制备的,并从含有NaCl和硫酸链霉素的无菌溶液进行冻干的。
分析说明
内皮细胞生长补充剂(ECGS)的增殖活性是通过使用低密度接种的胎牛心脏内皮细胞(ATCC CRL 1395)而在培养中进行测试的。EC50被定义为在基于细胞的生物测定中可引起细胞生长增加50%的生长因子有效浓度。
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
T Maciag et al.
Proceedings of the National Academy of Sciences of the United States of America, 76(11), 5674-5678 (1979-11-01)
Extracts of bovine hypothalamus were found to contain a significant level of mitogenic activity when tested in a Swiss 3T3 cell [3H]dThd incorporation assay and in a human umbilical vein endothelial cell growth assay. The mitogenic activity responsible for 3T3
Coculture of mesenchymal stem cells and endothelial cells enhances host tissue integration and epidermis maturation through AKT activation in gelatin methacryloyl hydrogel-based skin model
Zhang X, et al.
Acta Biomaterialia, 59(4), 317-326 (2017)
T Maciag et al.
The Journal of biological chemistry, 257(10), 5333-5336 (1982-05-25)
Endothelial cell growth factor (ECGF) is a neural-derived mitogen capable of stimulating the proliferation of quiescent populations of human umbilical vein endothelial cells (HUV-EC). ECGF has been isolated from bovine hypothalamus in two forms: a high molecular weight from (high
Heparin/endothelial cell growth supplement regulates matrix gene expression and prolongs life span of vascular smooth muscle cells through modulation of interleukin-1
Hsu JY, et al.
In Vitro Cellular & Developmental Biology. Animal, 35(10), 647-654 (1999)
Soluble adhesion molecules correlate with surface expression in an in vitro model of endothelial activation
Kjaergaard AG, et al.
Basic and Clinical Pharmacology and Toxicology, 113(4), 273-279 (2013)
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