E2884
酯酶 来源于猪肝脏
ammonium sulfate suspension, ≥150 units/mg protein (biuret)
别名:
PLE, 羧酸酯水解酶, 羧酸酯酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥150 units/mg protein (biuret)
Biological source:
Porcine liver
General description
酯酶，又称羧基酯水解酶，是水解酶超家族中的一员。
Application
从猪肝中提取的酯酶已用于揭示紫外可见光谱中的荧光。
猪肝酯酶被用于制药和工业应用中以催化五乙酰儿茶素和表儿茶素的水解。
猪肝酯酶常用于有机化学中的动力学拆分和不对称合成。
猪肝酯酶常用于有机化学中的动力学拆分和不对称合成。
该酶（来自 Sigma）已被用于开发一种基于凝胶的替代实验方案，用于水性介质中的珠结合催化活性的筛选。该产品还用于研究化合物 L-缬氨酸-3- {8- [（E）-2- [3-甲氧基苯基]乙烯基] -7-甲基-1-炔丙基黄嘌呤-3-基}丙基酯盐酸盐的性质（MSX-4，一种腺苷 A2A 受体拮抗剂 MSX-2 的氨基酸酯前药）。
Biochem/physiol Actions
细菌和真菌的酯酶具有多种工业应用。它能催化酯键的水解和合成。
猪肝酯酶催化酯的对映选择性转化，产生羧酸。分子量为 168kDa。它是一种丝氨酸酶，每个分子上有两个活性位点，它在稀酸或浓盐存在下解离成活性半分子。
酯酶作用于含有短链脂肪酸的水溶性羧酸酯。其功能来自于Ser、His和Asp/Glu的催化三联体。
Physical form
悬浮于 3.2M（NH4）2SO4 溶液，pH 8
Other Notes
一个酶活性单位是指在pH 8.0、25℃条件下每分钟将1.0 μM丁酸乙酯水解成丁酸和乙醇所需的酶量。
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
低风险生物材料
动植物源性产品
此项目有
Fluorogenic affinity label for the facile, rapid imaging of proteins in live cells
Watkins RW, et al.
Organic & Biomolecular Chemistry, 7(19), 3969-3975 (2019)
Karl Vollmann et al.
Molecules (Basel, Switzerland), 13(2), 348-359 (2008-02-29)
The compound L-valine-3-{8-[(E)-2-[3-methoxyphenyl)ethenyl]-7-methyl-1-propargylxanthine-3-yl}propyl ester hydrochloride (MSX-4) was synthesized as an amino acid ester prodrug of the adenosine A2A receptor antagonist MSX-2. It was found to be stable in artificial gastric acid, but readily cleaved by pig liver esterase.
Robert L Wilensky et al.
Nature medicine, 14(10), 1059-1066 (2008-09-23)
Increased lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) activity is associated with increased risk of cardiac events, but it is not known whether Lp-PLA(2) is a causative agent. Here we show that selective inhibition of Lp-PLA(2) with darapladib reduced development of advanced coronary
B Wang et al.
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With the discovery of an increasing number of biologically active peptides and peptide mimetics (1-3), there is a pressing need for the development of strategies to deliver these biologically active compounds to the desired site of action. The preceding two
F Dorandeu et al.
Toxicology, 248(2-3), 151-157 (2008-05-03)
Organophosphorus chemical warfare agents (nerve agents) are to be feared in military operations as well as in terrorist attacks. Among them, VX (O-ethyl-S-[2-(diisopropylamino)ethyl] methylphosphonothioate) is a low volatility liquid that represents a percutaneous as well as an inhalation hazard if
商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
实验方案
Objective: To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.
在测定酯酶活性时，使用丁酸乙酯通过pH滴定速率法进行测定。酯酶可以将酯水解成酸和醇。
相关内容
QC Methods
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| E2884-20KU | 04061833602980 |
| E2884-5KU | 04061832755588 |
| E2884-1KU | 04061833602973 |
| E2884-200UN | 04061832755564 |
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