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Merck
CN

E3004

提取-N-Amp™ PCR ReadyMix™

Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

别名:

Plant direct PCR master mix, Tissue direct PCR master mix

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关于此项目

NACRES:
NA.55
UNSPSC Code:
41106303

主要文件

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usage

sufficient for 100 reactions, 1.2 mL sufficient for 100 amplifications, sufficient for 1000 reactions, 12 mL sufficient for 1000 amplifications, sufficient for 10000 reactions, 125 mL sufficient for 10000 amplifications

feature

dNTPs included, hotstart, Direct PCR

Quality Level

200

technique(s)

PCR: suitable

color

colorless

input

crude DNA

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

相关类别

Application

Extract-N-Amp ™PCR ReadyMix™已被用于以下用途：
  • 基因组 DNA 提取
  • 真菌 DNA提取和聚合酶链反应（PCR）扩增
  • 直接 PCR 扩增
  • 基因分型
  • 实时PCR

General description

Extract-N-Amp™ PCR ReadyMix™是一种专门配制的热启动PCR预混液，可直接以提取物进行PCR扩增。
PCR ReadyMix 预期与 Sigma 的 Extract-N-Amp Plant PCR 试剂盒和 Extract-N-Amp Tissue PCR 试剂盒配套使用。 所有 Extract-N-Amp 试剂盒均包括 PCR ReadyMix，每次提取可充分进行一次 PCR 反应。但是，如果需要额外的 PCR 反应，可能需要补充 PCR ReadyMix。

Legal Information

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

低风险生物材料
常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000482390
0000482390
0000482391
0000482391
0000473006

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Muhammad Iqbal et al.
Genome, 50(5), 511-516 (2007-07-07)
Vernalization response (Vrn) genes play a major role in determining the flowering/maturity times of spring-sown wheat. We characterized a representative set of 40 western Canadian adapted spring wheat cultivars/lines for 3 Vrn loci. The 40 genotypes were screened, along with
Xuan Song et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 39(32), 6378-6394 (2019-06-14)
ATM (ataxia-telangiectasia mutated) is a PI3K-like kinase best known for its role in the DNA damage response (DDR), especially after double-strand breaks. Mutations in the ATM gene result in a condition known as ataxia-telangiectasia (A-T) that is characterized by cancer
Lieuwe Roorda et al.
BMC research notes, 4, 11-11 (2011-01-25)
In Dutch laboratories molecular detection of B. pertussis and B. parapertussis is commonly based on insertion sequences IS481 and IS1001, respectively. Both IS elements are more widely spread among Bordetella species. Both Bordetella holmesii, and B. bronchiseptica can harbour IS481.
Anneke van der Zee et al.
PloS one, 11(3), e0150755-e0150755 (2016-03-10)
The objective of our study was the development of a semi-quantitative real-time PCR to detect uropathogens. Two multiplex PCR reactions were designed to detect Escherichia coli, Klebsiella spp., Enterobacter spp., Citrobacter spp., Proteus mirabilis, Enterococcus faecalis, and Pseudomonas aeruginosa. 16S
Huirong Gao et al.
Frontiers in plant science, 11, 535-535 (2020-05-21)
Modern maize hybrids often contain biotech and native traits. To-date all biotech traits have been randomly inserted in the genome. Consequently, developing hybrids with multiple traits is expensive, time-consuming, and complex. Here we report using CRISPR-Cas9 to generate a complex
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商品

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Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Extract-N-Amp™ Tissue Feature

Extract-N-Amp™ Tissue PCR Kit quickly extracts PCR-ready DNA from mouse tails, providing optimized PCR mix for efficient amplification.

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