form
liquid
analyte chemical class(es)
proteins (Immunoglobulins of various mammalian species)
technique(s)
affinity chromatography: suitable
immunoprecipitation (IP): suitable
capacity
≥8 mg/mL, gel binding capacity (rabbit IgG)
storage temp.
−20°C
Quality Level
Application
在小规模亲和捕获中，如免疫沉淀，难以在微量离心管中看到亲和基质。 在移液时意外吸取树脂，会导致结果产生数量差异。EZview™ 红色亲和凝胶可大大降低沉淀损失的风险。 EZview树脂的性能与传统无色亲和凝胶相同，但更易于区分沉淀和上清液。 这将获得更准确的数据，因为蛋白质损失减少。
Features and Benefits
- 可见性增加-红色可降低意外吸入的风险
- 通过减少意外损失提高目标蛋白的回收率
- 重现性更高-收率更一致
General description
EZ view Red蛋白G亲和凝胶通过共价键与4%琼脂糖珠结合。该亲和凝胶用于将抗体IgG的FC部分与样品结合，如细胞裂解物和组织，纯化标记蛋白以进行免疫沉淀分析。红色染料增强了可视性，以获得更有效的结果。
Physical form
1:1（v/v）混悬液溶于含有50%甘油和15 ppm Kathon的PBS中。
PBS中50%悬浮液加入50%甘油及0.0015%
Legal Information
EZview is a trademark of Sigma-Aldrich Co. LLC
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
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